Qualitative and quantitative PCR (polymerase chain reaction) detection method of strain specificity of transgenic carnation Moonshade本发明公开了一种转基因香石竹Moonshade的品系特异性定性,定量PCR检测方法,包括以下步骤:1)转基因香石竹Moonshade基因组DNA的提取;2)转基因香石竹Moonshade外源基因插入位点的左边界旁...
Real-time PCRQuantitative PCRThe herbicide-tolerant transgenic rapeseed Topas 19/2 (synonym HCN92) has been approved for environmental release in Canada, Japan, Australia and the USA, and exported to a number of other countries as raw material. The purpose of this study was to establish event-...
SYBR green I; and Cy-5 labeled dUTP were used for real-time and end-point detection of specific PCR products. The efficiencies of both one-stage and two-stage reactions was shown to be strongly dependent on magnesium and primers concentrations. Quantitative PCR in the both versions was ...
Molecular analyses of early disease events require infected plant tissue in which the pathogen is present in high quantities and interacts with the plant in a way found in the field. In this study, a quantitative polymerase chain reaction (Q-PCR) assay was developed to determine all "infection...
Comparison of chromosome banding analysis, interphase- and hypermetaphase-FISH, qualitative and quantitative PCR for diagnosis and for follow-up in chronic... A sensitive (1蟽 rms at 1 MHz resolution ~3 mK) 1 mm spectral line survey (214.5-285.5 GHz) of VY Canis Majoris (VY CMa) and IRC...
The results revealed the LOD of 0.05% of GM maize for qualitative PCR assay and 4 transgenic haploid genome copies for quantitative PCR assay used in this study. The LOQ was 20 transgenic haploid genome copies, and based on this, as low as 0.05% of 98140 genomic DNA could be accurately ...
Quantitative multiplex detection of plant pathogens using a novel ligation probe-based system coupled with universal, high-throughput real-time PCR on Open... p pBackground/p pDiagnostics and disease-management strategies require technologies to enable the simultaneous detection and quantification of a ...
Subsequently, an equivalent of 500 pg RNA was used in a quantitative RT-PCR amplification in a MyiQ single-color real-time PCR system (Bio-Rad) with iQ SYBR Green Supermix (Bio-Rad) in a final reaction volume of 15 ml. Primers: Y51FWD: 5?-AGT TGG TCC GAG ...
Expression changes of nine HS-modifying enzymes in human fibrogenic diseases and HCC were measured by quantitative RT-PCR. Increased abundance and altered immunolocalization of HS was paralleled by elevated mRNA levels of HS-modifying enzymes in the diseased liver. The strong immunoreactivity of the ...
Southern-blot analysis revealed that Iiccr was a high-copy gene and real-time quantitative PCR... Yongsheng,Hu,Peng,... - 《Molecular Biology Reports》 被引量: 59发表: 2011年 A Quantitative Study of The Gibberellin Content of Seeds of Phaseolus Vulgaris at Different Stages in Their ...