Ntanet Website Status History The above graph displays service status activity for Ntanet.nic.in over the last 10 automatic checks. The blue bar displays the response time, which is better when smaller. If no bar is displayed for a specific time it means that the service was down and the...
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The supernatant was applied to 0.6 mL Ni-NTA agarose beads (GoldBio, H-350-50) and incubated on a rocker in a cold room for 1 h. Washing and elution steps were done in batch method. Beads were washed with 10 mL wash buffer for 10 mins then washed again with 10 mL ...
Pull-down assays To screen for interacting proteins of RsdA, total soluble proteins of P. fici and recombinant RsdA protein were incubated in vitro. In brief, 1 mg of RsdA-His protein was loaded onto Ni-NTA resin, and 5 ml wash buffer (50 mM NaH2PO4, 300 mM NaCl, 30 mM ...
Looks like the NOTICES part is down by ~700kB. acoutts commented Apr 30, 2021 Does this mean it's under the 10mb limit now to use in production? alex-zige commented May 4, 2021 @xster thanks for the #71102, looks like it's still just a bit over 10m for production? oantajam...
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The E. coli pellet was solubilized in 6 M urea in 50 mM Tri-Cl buffer, pH 8.0, then purified with a Ni-NTA column (GE Healthcare). Purified recombinant Nl Vg protein was used to immunize rabbits as described previously [54]. The sera of the immunized rabbits were collected as anti-...
His-fusion proteins were purified by using the Ni-NTA His-Bind Resin (Novagen), eluted with 0.5 M imidazole and dialyzed with BC-100 buffer. In vitro kinase assay. For in vitro kinase assay, recombinant human STAT1 (Biosource, Camarillo, CA, USA) and STAT2 (Abcam, Cambridge, MA, USA)...
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