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Nevada,nhetherand, with itsizzling, drydesertsndreezing,nowyountains—the last place ,ssociated withrchids—boasts noewerhanourteen nativerchids,wof ... C Siegel - 《Orchid Digest》 被引量: 0发表: 2010年 THE NATIVE ORCHIDS OF NEVADA PITY POORAWAII...ontraryo its lush imagesherchidapi...
m6A motif inMETTL16deficiency were mediated by the reduced intercellular SAM [46].Caenorhabditis elegans(C. elegans) METT-10 methylates a variant motif UACm6AGAAAC on the 3′ splice site (AG) of SAM synthetase (SAMS) pre-mRNA. The m6A on the 3′ splice site (AG) hinders the binding of...
The NAADP-protein complex was captured by Ni-NTA beads, washed three times with TBS, and eluted with 300 mM imidazole in TBS. For the assay involving 32P-NAADP binding to cell membranes, we followed a previously reported procedure6 with slight modifications. Cell membranes corresponding to 1...
(GE) to confirm the monomeric form of Gc. For purification of VSV-G, 4 days after transfection, cells were collected and sonicated with PBS. The cell lysate was centrifuged at 14,000×gfor 15 min and subjected to Ni-NTA Sepharose chromatography. The purified protein was dialyzed with ...
AzureJoined: Yes EnterpriseJoined: No DomainJoined: Yes DomainName: company domain Device Name: Computers name It shows as Hybrid Azure AD Joined in the AAD devices area. MDM shows as none.
ORF19 was produced in Sf9 cells and purified for lipase assay using Ni–NTA beads (Qiagen). Extraction was confirmed by western blot analysis using anti-ORF19 polyclonal antibodies (1:5000). Prediction studies All predictions were carried out on software available from http://au.expasy.org. ...
The supernatant was applied to 0.6 mL Ni-NTA agarose beads (GoldBio, H-350-50) and incubated on a rocker in a cold room for 1 h. Washing and elution steps were done in batch method. Beads were washed with 10 mL wash buffer for 10 mins then washed again with 10 mL ...
Pull-down assays To screen for interacting proteins of RsdA, total soluble proteins of P. fici and recombinant RsdA protein were incubated in vitro. In brief, 1 mg of RsdA-His protein was loaded onto Ni-NTA resin, and 5 ml wash buffer (50 mM NaH2PO4, 300 mM NaCl, 30 mM ...