What is the purpose of a salt bridge in a galvanic cell? Could you have a galvanic cell without it? What is the purpose of the salt bridge in a galvanic cell? Could you have a galvanic cell without it? Why would NaCl and KCl give off different colors when burned?
Nu: nucleophile. b Overlap of five solved structures from the GH3 family and modeled rBxTW1 showing the three conserved acid residues of the active site and a β-d-glucose ligand. E: Catalytic acid/base (Glu); D: Catalytic nucleophile (Asp); E/D: Conserved acid residue (Glu for β-...
C, biofilm phenotypes of a nonpolar ΔbcsG deletion in E. coli AR3110 functionally complemented by pBcsG or single alanine variants thereof. Replacement of the Zn2+-binding triad Cys243, Glu442, and His443; the catalytic nucleophile Ser278; His396; the Cys pair Cys290-Cys306; or the ...
Unlike protein tyrosine phosphatases, which generally utilize an intrinsic residue, such as Cys or Asp, as the nucleophile to attack the phosphate group, many Ser/Thr phosphatases require a metal ion to activate a water molecule to carry out the dephosphorylation reaction19. Divalent metal ions ...
After the induction period, cells were harvested and resuspended in HisTrap binding buffer (20 mm Tris, pH 8.0, 500 mm NaCl, and 40 mm imidazole) and lysed using a Constant System cell disruptor and centrifuged at 40,000 × g for 30 min. The cleared lysate was loaded onto a 5-ml ...
we repeated the force-quench experiments at pH=7.2 using the small thiol 3-mercapto-1-propanol as the attacking nucleophile41(Supplementary Fig. 11). In this case, the rupture of each individual disulfide bond resulted in the formation of a thiolate and the newly formed mixed disulfide. Unlike...