CLEM revealed that puncta near the chromosomes at telophase were surrounded by a double-membrane structure similar to the NE or ER (labeled dot 1 in Fig.3b, c). This NE-like membrane appeared to be fused with the NE on the chromosome (Fig.3c), suggesting that the transfected DNA may en...
BNIP3 and NIX induces the opening of a pore in the mitochondrial double membrane, suggesting that BNIP3 may have a critical role in the translocation of lysosomal proteins from cytoplasm into mitochondria during the MALM process.3 To examine the role of Mieap in intestinal tumorigenesis in vivo...
where they feed on bacteria [25]. They harbor small double membrane-bound organelles of unknown function which structurally resemble hydrogenosomes [25,26] but are probably derived from mitochondria [
In the intrinsic pathway, p53 modulates the bcl-2 family of proteins, including anti-apoptotic bcl-2 and pro-apoptotic bax, which releases cytochrome C from the mitochondria and activates caspase-9 followed by activation of caspase-3 [24,25]. Caspase-3 is recognized as a key member of ...
For analysis of the mitochondrial inner membrane potential (Δψm) in whole cells, the membrane-permeable lipophilic cationic fluorochrome JC-1 was utilised (Mitoscreen kit, BD Biosciences, UK). In live cells, JC-1 exhibits potential-dependent accumulation in mitochondria forming J-aggregates. The...
Because mitochondrial inner membrane polarization relies on efficient electron transport through respiratory complexes, we measured the activity of complex I (NADH-coenzyme Q oxidoreductase) (Fig.7a), the most important NADH oxidase activity in mitochondria, and found more than 50% decrease of the acti...
When mitochondria produce energy, they store electrochemical potential energy in the inner mitochondrial membrane. On both sides of the inner membrane, the asymmetric distribution of proton and other ion concentrations form MMP. Sperm motility is the main determinant of male fertility, and MMP is ...
Carnation Italian ringspot virus (CIRV) is a positive-strand RNA virus that causes massive structural alterations of mitochondria in infected host cells, the most conspicuous being the formation of numerous internal vesicles/spherules that are derived from the mitochondrial outer membrane and serve as...
Mitochondria are double-membrane organelles organized as an interconnected network52. The mitochondrial bioenergetic function is related to mitochondrial morphology31,53; and therefore impaired mitochondrial integrity can, in turn, affect their activity; leading to degenerative processes31,54. Since old mice...
To this point, we observed that the mitochondria proteome, particularly at the inner mitochondrial membrane (IMM) (cataloged in Mitocarta 2.016) is enriched for proteins smaller than 100 a.a. i.e. SEPs (p-value = 2.2×10−16) (Fig.1a–c), and speculated that mitochondria are a ...