Expression of a myelin proteolipid protein (Plp)-lacZ transgene is reduced in both the CNS and PNS of Plp(jp) mice. Neurochem Res 2007, 32: 343–351.Wight PA , Duchala CS , Shick HE , Gudz TI , Macklin WB ( 2007 ) Expression of a myelin proteolipid protein (Plp)-lacZ transgene ...
Fig. 2: MyoD is an anchor protein for chromatin loop formation in muscle cells. a Aggregate Peak Analysis (APA) plots showing the aggregated Hi-C contacts around chromatin loops identified in WT-GM and WT-DM cells. n represents the total number of chromatin loops called by HiCCUPS (see Met...
To distinguish between the expression of GPR37 in oligodendrocytes versus other cell types, we performed PCR with reverse transcription (RT–PCR) analysis of brain mRNA isolated from wild-type and R26;lacZbpA(flox)DTA mice, in which oligodendrocytes were eliminated using a binary genetic system27,...
acost breakdown 成本细分[translate] athe Sprn promoter, exon 1, intron 1 and the 50-end of exon 2 driving expression of either the LacZ or GFP[translate] aThe protein Shadoo (Sho) is a paralogue of prion protein, and encoded by the gene Sprn. Like prion protein[translate]...
Under a Creative Commons license Open accessThe vaccinia viral protein A27 in mature viruses specifically interacts with heparan sulfate for cell surface attachment. In addition, A27 associates with the viral membrane protein A17 to anchor to the viral membrane; however, the specific interaction between...
UBI4-LacZ expression was virtually identical in cells having constitutively high (ras2, sra1-13) or constitutively low (ras2) levels of cyclic AMP-dependent protein kinase activity, indicating that this kinase does not exert a major influence on UBI4 expression. Catabolite derepression control of...
A lacZ gene fusion from a positive clone was expressed in a λ-lysogen and yielded a protein of the expected size that was recognized by 37C12 on an immunoblot (Fig. 3 A). Thus, although full-length protein from a yeast nuclear extract did not react on immunoblots probed with 37C12 (...
A minimal human MBP Promoter-lacZ transgene is appropriately regulated in developing brain and after optic enucleation, but not in shiverer mutant mice (1998) A minimal human MBP promoter-lacZ transgene is appropriately regulated in developing brain and after optic enucleation, but not in shiverer ...
To test the assumption that CC3252 could control σF activity, we monitored the expression of σF-dependent genes in parental cells overexpressing CC3252 from a plasmid-encoded copy of the gene under the control of the constitutive lacZ promoter present in vector pJS14. For that, cells ...
a central helical domain and terminal, extended arms that clasp the compact ASCC2 unit. ASCC2–ASCC3 interfaces are evolutionarily highly conserved and comprise a large number of residues affected by somatic cancer mutations. We quantified contributions of protein regions to the ASCC2–ASCC3 ...