Uses of lacZ to Study Gene Function: Evaluation of β-Galactosidase Assays Employed in the Yeast Two-Hybrid SystemAuthor links open overlay panelIlya G. Serebriiskii, Erica A. Golemis 1Show more Add to Mendeley Share Cite https://doi.org/10.1006/abio.2000.4672Get rights and content...
Expression of the URA3 gene of Saccharomyces cerevisiae was studied by analysis of URA3-lacZ gene fusions constructed in vitro. Synthesis of hybrid β-galactosidase by fusions in frame with the coding sequence for orotidine-5′-phosphate decarboxylase (OMPdecarboxylase) was found to be normally ...
The enrichment of Set7/9 in β-cells appears to be dependent upon Pdx1, as knockdown of Pdx1 in INS-1 β-cells using small hairpin RNAs almost completely abolishes Set7/9 expression. A LacZ expression vector driven by the -6.5 kilobase pair Set7/9 promoter that contains putative Pdx1 ...
S1). E. coli Tuner DE3 pLacI strain was chosen as heterologous host as it does not display any endogenous β-galactosidase activity (due to the deletion of the LacZ gene) that may interfere with the enzymatic characterisation of the recombinant enzymes. The kinetic parameters of all GH29 ...
We investigated the effect of intrapenile injection of muscle-derived cells (MDC) on the erectile function in rats with bilateral cavernous nerve injury. Rat MDC were harvested and transduced with a retrovirus expressing the lacZ gene. Hanks' balanced salt solution (HBSS) (20l) or MDC (1 脳...
“Insertion of a lacZ trapping cassette and a floxed promoter-driven neo cassette … into the intron of a gene.”1Removal of the gene-trap cassette between FRT sites by FLP recombinase reverts the mutation to wild type, restoring gene activity, and leavesloxPsites on either side of the ...
1a). To understand the role of TaPIR1 in wheat–Pst interaction, we first employed barley stripe mosaic virus (BSMV)-induced gene silencing to knock down TaPIR1 expression. At 10 days after BSMV inoculation, photobleaching was observed in Phytoene desaturase (TaPDS)-silenced plants, which ...
The lac operon is an inducible operon that is comprised of lactose metabolizing genes, called lacZ, lacY, and lacA, that activate when lactose is present in the environment. Simply put, when the milk sugar lactose is present, the lac operon starts expressing genes to make lactose metabolizing ...
Catalase-peroxidase of Caulobacter crescentus: Function and role in stationary-phase survival.Examines physiologically the oxidative and starvation stresses in the gram-negative bacterium, Caulobacter crescentus, through a study of lacZ gene fusion and null mutant strains constructed from the cloned 5' ...
The LacZ gene replaces the connexin coding sequence in each of these null-mutant animals. Insets are antibody controls demonstrating WT sections are negative for the β-galactosidase marker. Scale bars, 50 μm. Full size image Differential expression of connexins in subsets of NPCs We used ...