Binding affinities of chemically modified heparins for human stem cell factor (SCF) were examined using fragmin/protamine microparticles (F/P MPs) and an enzyme-linked immunosorbent assay (ELISA). The binding of SCF to F/P MP-coated plates was inhibited with high concentrations of heparin and ...
can be excluded, because the alkaline phosphatase is diminished by 2.8% and 8% and also the phosphate-clearance by 16.5%.鈥擳he large elevation of inorganic phosphate may be caused by further enhancement of the disturbance of oxydative phosphorylation by the enzyme-restricting effect of heparin. ...
Heparin has been used clinically as an anti-coagulant for more than 100 y and the major source of this therapeutic is still animal tissues. Contamination i... MS Lord,MS Jungy,JM Whitelock - 《Bioengineered》 被引量: 0发表: 2017年 Heparin in the Treatment of Burns Burns are difficult to...
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This was followed by washing the cells three times with L15 supplemented with heparin (Sigma-Aldrich) (1 mg/mL) and an extra L15 wash. Cells were treated with the cell impermeable nuclear stain SYTOX Blue (Thermo Fisher Scientific), SYTOX Green (Thermo Fisher Scientific), or DRAQ 7 (...
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reduced levels in several mammary tumor cell lines. The mitogenic activity of purified AR was also shown to be inhibited by heparin sulfate, and an AR-specific enzyme-linked immunosorbent assay (ELISA) revealed that KAF and AR are antigenically related. We have previously shown that human ...
The mitogenic activity of purified AR was also shown to be inhibited by heparin sulfate, and an AR-specific enzyme-linked immunosorbent assay (ELISA) revealed that KAF and AR are antigenically related. We have previously shown that human keratinocytes can grow in an autocrine manner. Our present...
A particular region known as the heparin-binding site has been suggested as the origin of FGF2 instability. A large body of evidence has shown that binding of heparin or heparan sulfates (HS)—in particular heparan sulfate proteoglycans (HSPGs)—stabilizes the FGF2 structure and function via ...
(SAM), the universal methyl donor for all methylation reactions33. The nuclear-encoded mitochondrial enzyme MUT uses B12as a cofactor for the catabolism of branched-chain amino acids via isomerization of methylmalonyl-CoA to succinyl-CoA, for use in the tricarboxylic acid cycle (Extended Data Fig...