2) HCl and KOH 3) {eq}H_2CO_3 and NaHCO_3 {/eq} 4) KCl and KOH Buffer Solutions: Buffer solutions are able to maintain the pH of a solution within a constant range as the small additions of acid or base is consumed by the comp...
Answer to: Calculate the pH of a 0.20 M NH_3/0.20 M NH_4Cl buffer. What is the pH of the buffer after the addition of 10.0 mL of 0.10 M HCl to 65.0...
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While the accessibility of enhancers is dynamically regulated during development, promoters tend to be constitutively accessible and poised for activation by paused Pol II. By studying Lola-I, a Drosophila zinc finger transcription factor, we show here t
Cells were incubated in quenching solution (100 mM Glycine in PBS-CM, pH 7.5) twice for 10 min, washed in PBS-CM pH 7.5 and then scraped and lysed in lysis buffer (20 mM Tris–HCl pH 8.0, 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 0.2% BSA, plus protease inhibitors...
4. Buffer 50 mmol/L, pH 7.8 Tris HCl 5.32 g Tris Base 1.97 g Sterile water 1000 mL The pH is checked at 25 C and adjusted if necessary.Factor X activating enzyme from Russell's Viper Venom; isolation and characterisation. Purificationand partial characterisation of activated decarboxyfactor ...
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Samples were spun at 15,000 × g, and supernatants adjusted to 190 mmol/L NaCl, 50 mmol/L Tris-HCl, pH 8.3, 6 mmol/L ethylenediaminetetraacetic acid, and 2.5% Triton X-100 and incubated overnight with antibody 4G8 (Signet Laboratories), followed by addition of secondary rabbit anti-...
Cells were harvested and frozen until used. After suspension in 50 mm Tris/HCl, pH 7.5, with 10% (v/v) glycerol, the thawed cells were lysed by a single pass through a French pressure cell at 20,000 p.s.i. Cell debris was removed by centrifugation at 30,000 × g for 60 min, ...
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