NEBuffer™ r2.1: 25% NEBuffer™ r3.1: 50% rCutSmart™ 缓冲液: 100% 稀释兼容性 稀释液 A 贮存溶液 10 mM Tris-HCl 50 mM KCl 1 mM DTT 0.1 mM EDTA 200 µg/ml Recombinant Albumin 50% Glycerol pH 7.4 @ 25°C 热失活 否 甲基化敏感性 dam 甲基化: Blocked by Ov...
NEBuffer™ r2.1: 25% NEBuffer™ r3.1: <10% rCutSmart™ 缓冲液: 100% 稀释兼容性 稀释液 A 贮存溶液 10 mM Tris-HCl 50 mM KCl 1 mM DTT 0.1 mM EDTA 200 µg/ml Recombinant Albumin 50% Glycerol pH 7.4 @ 25°C 热失活 否 甲基化敏感性 dam 甲基化: Not Sensitive...
How does the level of star activity of SalI-HF compare to SalI? What is the difference between SalI-HF and SalI? Why does the HF version of the enzyme have a different recommended buffer than the wild type enzyme? When should I choose the HF version of an enzyme? When is star activity...
单位活性检测条件 25 mM TAPS-HCl(25℃ 时 pH 9.3)、50 mM KCl、2 mM MgCl2、1 mM β-巯基乙醇、200 µM dNTP(含 [3H]-dTTP)和 15 nM 已结合引物的 M13 DNA。 优势和特性 Features 稳健的反应体系 – 最少的优化工作伴随最大的成功可能性 超高保真度 –是Taq酶的 50 倍 快速 – 显著缩短延伸...
Buffer Not a buffer Buffering Solution: A buffering solution maintains a constant pH by eliminating the effects of a change in the concentration of H+ ions. This is achieved through two equilibrium reactions. Answer and Explanation:1 Buffers require a w...
Which set of compounds would form a buffer in an aqueous solution? (a) HF and KF (b) HCN and NaF (c) HCl and KCl (d) NaCl and NaOH (e) NaBr and KBr (f) NaF and KF (g) HCN and NaCN.What is the Delta H of HF (hydrogen fluoride)?
BalbBuffer 1.1: 25% BalbBuffer 2.1: 100% BalbBuffer 3.1: 25% CutSmart Buffer: 100% 特性: CutSmart、重组酶、基因工程改造酶、省时酶、高保真酶。 反应条件: CutSmart 缓冲液,37℃。 热失活:65℃ 20 分钟。 浓度: 20,000和100,000units/ml。 甲基化敏感性: 对哺乳动物基因组DNA CpG甲基化敏感。
1. Buffer busy waits 从本质上讲,这个等待事件的产生仅说明了一个会话在等待一个Buffer(数据块),但是导致这个现象的原因却有很多种。 常见的两种是: · 当一个会话试图修改一个数据块,但这个数据块正在被另一个会话修改时。 · 当一个会话需要读取一个数据块,但这个数据块正在被另一个会话读取到内存中时。
The cells were harvested by centrifugation and the cell pellet was frozen at –20°C. Next, the thawed pellet was re-suspended in lysis buffer (PBS (140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.3) containing 350 µg ml–1 Lysozyme, 20 µg ml–1 DNase, 20 µg ...
Aorta was cannulated and the coronary arteries retrogradely perfused in a modified Langendorff setup with buffer A (in mM: Hepes 25, NaCl 130, KCl 5.4, NaH2PO4 0.4, MgCl2 0.5, D-glucose 22, pH 7.4) at 37° C. for 2-4 min, then with buffer A containing 0.8 g/L collagenase II (...