Is GAPDH a relevant housekeeping gene for normalisation in colorectal cancer experiments? Br J Cancer 2010;103:1475-1476.Caradec J, Sirab N, Revaud D, Keumeugni C, Loric S (2010) Is GAPDH a relevant housekeeping gene for normalisation in colorectal cancer experiments? Br J Cancer 103 : ...
(I) 用不同浓度的LBH589处理后,眼部黑色素瘤细胞(92.1和CRMM1)中m6A相关蛋白相对于GAPDH的Western blot。*P<0.05,**P<0.01,***P<0.001。 缩写: RNA-seq:RNA测序;GO:Gene Ontology;m6A:N6-甲基腺嘌呤;IGV:综合基因组学查看器;No.:编号;TSS:转录起始位点。METTL14:甲基转移酶样14;METTL3:甲基转移酶样3;W...
aScheme of the LDLR protein showing the N-terminal LBD composed of seven CR repeats, a cluster of EGF modules containing a β-propeller domain, and a membrane-proximalO-linked glycans domain. SP: signal peptide; TM: transmembrane region.b–dHEK 293T cells stably expressing pig LDLR or mutan...
Moreover, the oxygen-sensitive HIF1α-AS1 is down-regulated in pulmonary hypertension and loss-of-function approaches not only result in gene de-repression but also enhance angiogenic capacity. As exemplified here with HIF1α-AS1, DNA:DNA:RNA triplex formation is a functionally important mechanism...
It was carried out in the Agilent Mx3005P System (Agilent Technology) using glyceraldehyde 3-phosphate dehydrogenase gene (GAPDH) as a reference gene. The specific primers for QRT-PCR of Cstps1 were designed according to the report of Sharon-Asa et al. (2003) [5]. The results of ...
For each time point, three PCR reactions were run and the average value was used for the calculation of relative expression using the ΔΔct method with GAPDH as a calibrator. Data are expressed as fold difference compared to the time zero hour. Graph shows the means and SD from three ...
MiR-21 expression was normalized to that of U6 small nuclear RNA, and PPM1D and PTEN expression was normalized to that of GAPDH. The expression of miR-21, PPM1D, and PTEN were defined based on the threshold cycle (Ct); relative expression levels are presented as 2–ΔΔCt....
were analyzed using ImagePro 5.0 (Media Cybernetics, Inc., Silver Spring, MD, USA) and normalized to the protein level of β-actin or GAPDH. Statistical analysis The results were presented as means ± S.E.M. and analyzed using one-way ANOVA followed by Tukey’s post-hoc test for ...
GAPDH CAACGGATTTGGTCGTATTG GATGACAAGCTTCCCGTTCT Immuno-gold labeling in transmission electron microscopy HeLa cells were seeded on 3 mm carbon-coated Sapphire disks (Wohlwend GmbH, Switzerland) at a density of 4000 cells/mm2 and allowed to settle for 12–18 h. The cells were subsequently...
Housekeeping gene GAPDH or β-actin was used as internal control for gene expression normalization. Pharmacological and genetic modulation of AR signaling AD LNCaP cells were cultured in regular FBS-containing medium. In this study, we employed several complementary approaches to modulate the cellular ...