agalactiae and they highlight the direct role of GAPDH in this process. Taken together, our data demonstrate that S. agalactiae GAPDH is a virulence-associated immunomodulatory protein. 展开 关键词: Proteins rGAP Laboratory mice virulent characteristics B-Lymphocytes 蛋白质类 B淋巴细胞 DOI: ...
protein unfoldingGAPDHIt is proposed that conformational changes induced in proteins by oxidation can lead to loss of activity or protein aggregation through exposure of hydrophobic residues and alteration in surface hydrophobicity. Because increased oxidative stress and protein aggregation are consistently ...
Knockdown of E7 by siRNA against E7 in HPV16-positive CaCx cell line, SiHa.ATaqMan-based qRT-PCR revealed 69% knockdown in HPV16 E7 expression in transfected group (T-SiHa) compared to the untransfected (UT-SiHa).BUpper panel: Western blot showing knockdown of E7 protein (19KDa) in trans...
(1:1,000). gapdh was used as a protein-loading control. the immune complexes were detected by chemiluminescence (ecl). rna immunoprecipitation (rip) assay rna immunoprecipitation was conducted using magna rip rna-binding protein immunoprecipitation kit (millipore) following the manufacturer's protocol...
A total of 64 proteins were identified that belong to different functional groups such as ribosomal and histone proteins as well as cytoskeleton proteins. The subcellular distribution of some of the proteins identified such as GAPDH and RPL18 was altered in Huh-7 infected cells. Since RPL18 was...
The total RNA was purified using MiniBEST Universal RNA Extraction Kit (Takara, Tokyo, Japan). RT-qPCR was performed using a TOPreal™ One-step RT-qPCR Kit (Enzynomics, Daejeon, South Korea). Our studies followed the manufacturer’s protocol. GAPDH primer was synthesized, and other primers ...
LC3/ATG8 has long been appreciated to play a central role in autophagy, by which a variety of cytoplasmic materials are delivered to lysosomes and eventually degraded. However, information on the molecular functions of LC3 in RNA biology is very limited.
Protein acetylation plays a key role in regulating cellular processes and is subject to aberrant control in diverse pathologies. Although histone deacetylase (HDAC) inhibitors are approved drugs for certain cancers, it is not known whether they can be de
Using bacterial cell lysate that contained overexpressed Yca1 protein, Silva et al. (22) identified glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a putative Yca1 substrate. Yca1 does not appear to directly interact with Bir1 or the yeast Omi/HtrA homologue Nma111 (4). Show abstract ...
a Results from A1 noradrenergic nucleus; b A2 noradrenergic nucleus and c A6 noradrenergic nucleus. Data are normalized to GAPDH and expressed as a fold change from regular chow-fed DIO group. * indicates significant difference from the corresponding DIO group (p < 0.05). 6–8 rats/group...