Gut microorganisms are solely responsible for the reduction of bilirubin to urobilinogen, potentially using the molecule as a terminal electron acceptor in anaerobic respiration. Despite the recognized role of gut microorganisms in bilirubin reduction, the gut microbial enzyme that reduces bilirubin to ur...
In addition, a set of the wild-type M3-type GAS strains MGAS158, MGAS274, MGAS315, MGAS335, MGAS1313, and MGAS2079 was also used. GAS cultures were routinely grown on brain-heart infusion agar (BD Biosciences) and in Todd-Hewitt broth (BD Biosciences) supplemented with 0.2% yeast ...
Brain Heart Infusion C. acnes : Cutibacterium acnes CFU: Colony Forming Unit ConA: Concanavalin A CSLM: Confocal Scanning Laser Microscopy DMEM: Dulbecco’s Modified Eagle Medium FBS: Fetal Bovine Serum MOI: Multiplicity of infection MSC: Mesenchymal stem cell PBS: Phosphate Buffered...
isolates is better on potato dextrose agar with chloramphenicol than on brain heart infusion agardoi:10.1016/j.mycmed.2009.04.002S. AnaneO. ChtourouA. ChediE. KaouechS. BelhadjK. KallelE. ChakerRitorno al numero
H. influenzae strains were grown as described previously (21) and were stored at −80 °C in brain-heart infusion broth with 20% glycerol. Escherichia coli strain DH5α was grown on Luria-Bertani (LB) agar or in LB broth. E. coli strains were stored at −80 °C in LB broth ...
Strains stored at − 80 °C in Brain Heart Infusion (BHI) broth (BD Difco) glycerol 15%, v/v (Carlo Erba)). They were first incubated overnight in 5 mL of BHI broth, and subsequently, BHI agar plates were streaked with the overnight cultures and incubated for 24 h. Few isolated...
If you’ve ever witnessed the process of death via brain cancer for instance, you’d catch my drift. I had an aunt die this way and it’s amongst the most disturbing ways you could ever contemplate your last days on earth. Now my mom has lung cancer and they’ve pissed of the wrong...
The matrix ofListeria monocytogenesbiofilms is composed of EPS, namely eDNA, proteins and polysaccharides46. Following the previous set of results, we investigated the macromolecular biofilm matrix composition of the wild-type, ΔpnpA,and complemented strains to understand if the observed differences in...
is primarily composed of brain microvascular endothelial cells (BMECs), basal lamina, astrocyte end-feet and pericytes7. During the past decades, in vitro cultured BMECs have been developed to study the invading mechanisms of CNS-infecting pathogens through the BBB. InE. coli, several virulence ...
In order to follow the growth of bacteria at subinhibitory concentrations of the active compounds at 37°C [13], 4 tubes (control, MIC, MIC/2, and MIC/4) were prepared, each containing 8 mL of Brain Heart liquid medium (BH), 1 mL of bacterial suspension, and 1 mL of the ...