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ISG15 is an interferon-stimulated, ubiquitin-like protein that can conjugate to substrate proteins (ISGylation) to counteract microbial infection, but the underlying mechanisms remain elusive. Here, we use a virus-like particle trapping technology to ide
1995) was maintained on brain heart infusion agar (Difco, Detroit, Mich.). After incubation for 3 days at 30°C, the plates were stored at 4°C. The 31-kDa alasan protein was obtained from alasan by preparative SDS-PAGE as described previously (Toren et al. 2001). Peptide analyses ...
A. muciniphila(ATCC BAA835) was cultured under anaerobic conditions at 37 °C in Brain Heart Infusion media supplemented with 0.05% hog gastric mucin-type III (Sigma-Aldrich, St. Louis, MO, USA) [29].A. muciniphilawas freshly cultured in anaerobic conditions as described above for about ...
Vibrio strains were cultured in marine 2216E broth (or agar) or tryptic soy broth with 2% NaCl (TSB+) at 28°C. Escherichia coli β2155 was cultured in Luria Bertani (LB) broth at 37°C, and it was also cultured in Brain Heart Infusion (BHI) Medium at 37°C. Bacterial strains ...
isolates is better on potato dextrose agar with chloramphenicol than on brain heart infusion agardoi:10.1016/j.mycmed.2009.04.002S. AnaneO. ChtourouA. ChediE. KaouechS. BelhadjK. KallelE. ChakerRitorno al numero
(Coy Laboratory Products). Strains were grown in 200 ml of liquid brain–heart infusion (BHI) broth (Research Products International, B11000) or yeast casitone fatty acid broth with 4.4 mg per 100 ml sterile filtered bilirubin dissolved in dimethylsulfoxide (DMSO, Sigma-Aldrich). The ...
Brain Heart Infusion C. acnes : Cutibacterium acnes CFU: Colony Forming Unit ConA: Concanavalin A CSLM: Confocal Scanning Laser Microscopy DMEM: Dulbecco’s Modified Eagle Medium FBS: Fetal Bovine Serum MOI: Multiplicity of infection MSC: Mesenchymal stem cell PBS: Phosphate Buffered...
enterocolitica strains were grown in Porcine Brain Heart Infusion (BD, Heidelberg) supplemented with 0.5% yeast extract and adjusted to pH 5.5. For maximal production of YadA, bacteria were also grown in RPMI 1640 without phenol red (Life Technologies, Darmstadt). Antibiotics were used at the ...
Proper peptone water dilutions of the meconium samples were plated in triplicate onto brain heart infusion (BHI, Oxoid, Basingstoke, UK), violet red bile agar (VRBA; Difco, Detroit, MI), and Columbia nadilixic acid agar (CNA, BioMerieux, Marcy l'Etoile, France) agar plates, which were ...