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Later, Dr. Brenner proposed that individuals with a reduced number of nephrons (whether due to genetic, environmental, or developmental factors) are more prone to kidney disease. Brenner’s research highlighted that individuals with fewer nephrons had to compensate by hyperfiltration (increased workl...
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For example, new evidence for neuroscientific or bioacoustical theories can change which assumptions we should rely on in our theories regarding the evolution of human symbolic communication; developments in those related scientific fields may require updating our theories. Evolution is a phenomenon about...
Real-time PCR was performed with Fast SYBR Green Master Mix on a Light Cycle 96 (Roche Life Science) or CFX (Bio-Rad) Real-Time PCR system. The PCR primers used are described in Table S6. The relative amounts of PCR products generated from each primer set were determined on the basis...
Most of the ongoing projects aimed at the development of specific therapies and vaccines against COVID-19 use the SARS-CoV-2 spike (S) protein as the main target. The binding of the spike protein with the ACE2 receptor (ACE2) of the host cell constitutes
Next, we washed them with FACS Buffer and then stained them for 15 min with PE Donkey anti-rabbit IgG (#406,421, BioLegend). Finally, we analyzed the cells using a BD FACSMelody Cell Sorter. Time-lapse photography We disaggregated endometrial tissue specimens donated by hysterectomy patients ...
(1991) Colonisation of biofilms by bacteria capable of biodegrading sodium dodecyl sulphate (SDS) at clean and polluted riverine sites. Microb. Ecol. 22, 85–98. Article Google Scholar Saye, D.J., Ogunseitan, O., Sayler, G.S. and Miller, R.V. (1987) Potential for transduction of ...
After washing with TBST, membranes were incubated with ECL-reagent (BioRad, cat. no. 1,705,061) according to the manufacturer’s protocol. Membranes were developed using standard X-ray films (AGFA, cat. no. EWPJH) or the chemiluminescence imaging system (Alliance Q9 Advanced, Uvitec, ...