unchanged, the electrostatic interactions should be weakened. We reasoned that this would generate a dilute arm with a more convex shape, and this is precisely what we observe (Fig.2e). Similarly, we performed simulations of a positively charged variant of FUS-LCD that was prescribed to have t...
Arginine (DR) residues at position 166–167 mutated to Alanine, Alanine (AA). The bar heights are based on the mean ofn = 6 independent biological replicates used to generate the plots and statistics ind. Error bars indicate 1 s.e. above and below the mean values.Pvalues of ...
GPVI has a positively charged arginine in its transmembrane region that is essential for association with the FcR γ-chain.45,46 A transmembrane arginine is also found in other receptors that associate with the FcR γ-chain including FcγRI, FcγRIII, FcϵRI, FcαR, PIR-A, and NKR-...
To support our concept that lysines are effective through their positive charge, we generated N1•PB2>R in which the three lysine residues in PB2 were mutated to positively charged arginines. Indeed, N1•PB2>R underwent LLPS similarly to N1 (Fig. S4A). In contrast, mutation of the ...
- Histones, on the other hand, are proteins that are rich in basic amino acids such as lysine and arginine. These amino acids have side chains that carry a positive charge. Step 2: The Interaction between DNA and Histones- In eukaryotic cells, DNA is organized into a structure called ...
Evidence comes from the accessibility to external methanethiosulfonate (MTS) reagents of the positively charged arginine residues (R) on this segment. These are first mutated to cysteines (C), which in turn react with MTS reagents; it is assumed that the C is passively carried in the S4 ...
One example is the Lysine- Arginine Rich Unidentified Function (KRUF) family of proteins [10] (Additional file 1: Table S3). Twelve of the 14 KRUF family members are strongly expressed in tachy- zoites but not expressed (≥8-fold lower) in merozoites. Genome annotation RNA-Seq data ...
Overview of YejM structure, constructs, and phylogenetic connection of YejM to selected members of the larger hydrolase superfamily. (a) Overview of YejM structure. The N-terminal transmembrane domain containing 5 helices (5TM) and linker region with positively charged arginine residues (RR) are ...
Conserved lysine and arginine clusters from both MID and MHF contribute to binding of the MID-MHF com- plex to HJ DNA The previous studies have used dsDNA-based bind- ing assays to confirm the importance of the predicted Cell Research | Vol 24 No 5 | May 2014 David Fox 3rd et al. ...
The three continuously conserved arginines (Arg68, Arg69, and Arg70) are located together in the α3 helix of LC3B, which may form a local positively charged area and serve as a potential protein-protein interaction sites. As mentioned above, only Arg68 can affect the cleavage of human Atg...