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We also immunostained with an NG2 antibody to probe for OPCs, which have been suggested to aid the repair of damaged myelin in conditions of CNS injury and neurodegeneration17,56. We observed a significant decrease in the percent OPC coverage area in the hippocampus of PS19-fE4 mice as co...
coli can be performed by use of nucleic acid probes for the detection of nucleic acids encoding Tir polypeptide, or by detecting the protein using an antibody which binds to Tir. A kit for detecting a Tir-producing E. coli and the antibody that binds recombinant methods, the Tir for ...
Among the many induced cytokines, we identify IFN-γ as an indispensable factor for inhibiting lung metastasis. Based on CyTOF (mass cytometry or cytometry by time of flight) analysis of the immune responses after Salmonella treatment and antibody-mediated cell depletion, we further demonstrate that...
This inhibitory immune response was abolished by an antibody-mediated transient in vivo depletion of gamma delta T cells. Two gamma delta T-cell clones were derived from malaria-immunized alpha beta T-cell-deficient mice. The adoptive transfer of one of these gamma delta T-cell clones to ...
As the HCMV antibody IgM represents a recent infection, an acute HCMV infection might affect the expression of CD14+CD16+ monocytes[50]. Our findings revealed that the CD14+CD16+ mononuclear cells displayed features that are typical of mature and inflammatory functions, and thus the high ...
1A). To further verify the presence of ANGPTL3 in HDL, co-immunoprecipitation assays with anti-ANGPTL3 antibody were performed on the HDL lysate of non-diabetic subjects. The immunoprecipitation complexes were examined by western blot analysis using the anti-ANGPTL3 antibody. A robust ANGPTL3 ...
The specificity of each antibody was confirmed by Western blot analysis of extracts from 293T cells transfected or not with the relevant complementary DNAs. The data presented are representative of at least three independent experiments. Expression at the protein level was confirmed by SDS-PAGE/...
5b,c). Notably, SA-β-gal+ cells were also PD-L2+ using IHC, further confirming that PD-L2 is a marker of TIS (Fig. 5d). To characterize the antitumor effect of combined chemotherapy and PD-L2 antibody, we analyzed lymphocytic infiltration using IHC in residual tumors at 13 weeks of ...
Fig. 2: Antibody responses elicited by SARS-CoV-2 vaccination. a,b, Antibody responses were studied in three groups of individuals (n = 24 per group) receiving primary vaccination with either BNT162b2, ChAdOx1 or mRNA-1273 by MSD-ECL assay (a) or pseudotype-based neutralization assay...