Here we identify a metallopeptidase, angiotensin-converting enzyme 2 (ACE2), isolated from SARS coronavirus (SARS-CoV)-permissive Vero E6 cells, that efficiently binds the S1 domain of the SARS-CoV S protein. We found that a soluble form of ACE2, but not of the related enzyme ACE...
If GETV uses LDLR as a host cell receptor, the soluble LDLR-LBD should be able to inhibit GETV infection. To test this hypothesis, we performed in vitro neutralization assays using rGETV-EGFP virions and purified GST-LBD. We found that GST-LBD, but not GST, neutralized the infectivity o...
but the mechanisms contributing to IL-27’s suppressive effects are still poorly understood. Takahashiet al. identified the gene encoding cholesterol 25-hydroxylase (Ch25h), the enzyme that converts cholesterol to 25-hydroxycholesterol (25OHC), as strongly and ...
光是叶绿体发育的必要条件, 可被一系列能够感 受特定波长的蛋白所感知, 蛋白通过构象变化与下游 信号分子相互作用.感受红光和远红光的光敏色素 (phytochrome A/B, phyA/B)及感受蓝光和紫外光的 隐花色素(cryptochrome, cry)是负责调控光形态建成 的两类光受体(Jiao et al., 2007).光敏色素相互作用 因子(...
Aryl hydrocarbon receptor, Cytokine (IL-6, TNF, IL-1β) response INTRODUCTION Cadmium (Cd) is a heavy metal widely spread in the environment[1, 2], which represents a significant water and food contaminant, and is among the most toxic metals in the environment. The long biological half...
cells expressing S protein. Furthermore, SARS-CoV replicated efficiently on ACE2-transfected but not mock-transfected 293T cells. Finally, anti-ACE2 but not anti-ACE1 antibody blocked viral replication on Vero E6 cells. Together our data indicate that ACE2 is a functional receptor for SARS-...
The best-studied germinant receptor is GerA from B. subtilis. It is encoded from a tricistronic operon during late sporulation by a σG promoter [201,202]. Each cistron encodes a unique subunit of the completed receptor and is named in the order of their transcription (gerAA, gerAB, and...
First, we generated an in vitro model of synucleinopathies in primary striatal cultures, in which moderate overexpression of aSyn resulted in mild mitochondrial dysfunction. Neurons were infected with lentivirus encoding human WTaSyn, A30PaSyn, or GFP, as control protein. To test whether expression...
@burcinc-- E stands for enzyme and S stands for substrate. When a substrate (molecule which the enzyme breaks down) attaches to an enzyme, it is called the enzyme-substrate complex, or ES. So you know that competitive inhibition is when an inhibitor binds itself to the same place on bot...
tPA is a serine proteinase, which cleaves the plasminogen to active plasmin, an enzyme responsible for blood clot breakdown.42 In the brain, tPA is found in association with an endothelial cell–astrocyte complex, where it modulates cerebrovascular tone, functioning at low doses as vasodilator and...