IRF3 and IRF7 require SIRT1 for liquid–liquid phase separation and transactivation of IFN-1Medical and Health SciencesImmunologyDeacetylation of specific lysine residues in the DNA-binding domain of the transcription factors IRF3 and IRF7 by SIRT1 is necessary for liquid–liquid phase separation and...
Deacetylation of specific lysine residues in the DNA-binding domain of the transcription factors IRF3 and IRF7 by SIRT1 is necessary for liquid–liquid phase separation and transactivation of type I interferons. SIRT1 agonists partially restored the impa
病毒与宿主IFN系统的互作决定了感染发生及疾病进程,因而病毒进化出多种策略来逃避或控制宿主抗病毒免疫反应。STING分子作为一个支架蛋白能够特异性的促进IRF3和TBK1的磷酸化,在FN释放中发挥着重要的作用。 禽黄病毒(Tembusu Virus,TMUV)属于黄病毒科黄病毒属的蚊媒病毒,该属许多成员是重要传染病病毒,如登革热病毒、日本...
Collectively, our study highlights PGAM5 as an important regulator for IFNβ production mediated via the TBK1/IRF3 signaling pathway in response to viral infection.doi:10.1038/s41598-020-65155-1Yu-qiang YuMarta ZielinskaWei LiDominic B. Bernkopf...
目前,人们普遍认为固有免疫和适应性免疫在肿瘤免疫监视中起重要作用,Ⅰ型干扰素(interferon,IFN)在连接固有免疫和适应性免疫引发有效的抗肿瘤免疫的过程中至关重要,因为抗原呈递树突状细胞(DC)产生的Ⅰ型干扰素在肿瘤微环境中,不仅激活固有免疫反应,还促进T细胞交叉激活和浸润[1]。大量研究表明细胞溶质双链DNA在各种细胞...
ELISA 检测Kupffer 细胞培养上清液中干扰素（IFN ）β、肿瘤坏死因子（TNF ）-α和白细胞介素（IL ）-1β含量。结果LXR α蛋白质表达水平在T0901317处理组最高，LPS 处理组最低。联合处理组中，LXR α表达明显低于T0901317处理组（P <0.05），但又显著高于其他两组（P <0.05）。IRF3及GRIP1蛋白表达量在LPS...
Thus, the WTAP-IRF3/IFNAR1 axis may serve as negative feedback pathway to fine-tune the activation of IFN-I signaling to protect the host from immunopathology of overreactive and harmful IFN-I production, which highlights the sophisticated regulatory mechanisms of antiviral responses at post-...
Our study identifies SMYD2 as a negative regulator of IFN-I production against virus infection. The new way of regulating IRF3 phosphorylation will provide insight into the understanding of IFN-I production in the innate response and possible intervention of the related immune disorders....
HSV-1接种,通过RT-q PCR和Western Blot检测,发现沉默IRF3后抑制PBLD上调IFNβ和部分ISGs的表达,表明PBLD促进的Ⅰ型干扰素反应依赖于IRF3.成功构建IRF3发生磷酸化的关键位点S385/386和S396位的突变体表达质粒,将其以及野生型的IRF3分别与PBLD共同转进敲除IRF3的He La细胞系中,用BPIV3,VSV,HSV-1接种,通过Western ...
In this study, we found an acetyltransferase KAT8 in grass carp (CiKAT8, MW286472) that acetylated IRF3/IRF7 and then resulted in inhibition of IFN 1 response. CiKAT8 expression was up-regulated in the cells under poly I:C, B-DNA or Z-DNA stimulation as well ...