Results : We observed that MacTel iPSC-RPE had elevated expression of enzymes in the serine synthesis pathway, and reduced levels of serine (21%) and glycine (15%) secretion indicating a defect in serine and glycine metabolism. Phagocytosis of POS and secretion of VEGF and PEDF were ...
视网膜变性疾病(RD),例如色素性视网膜炎(RP)、Stargardt病和老年人黄斑变性(AMD),致盲的主要原因是由于RPE细胞功能受损和感光细胞变性。健康的RPE细胞能分泌滋养感光细胞的PEDF因子等物质,挽救受损的感光细胞,就能逆转RD疾病的进程。 这就是为何RD疾病最有希望的治疗性策略之一是利用干细胞所培育的iPSC-RPE细胞来替换衰...
在过去的研究中,研究人员采用了原代细胞培养的方法来分化IPSC为RPE细胞。这种方法的优点在于可以直接从组织中获得RPE细胞,并且维持了RPE细胞的天然特性。但是,原代细胞培养方法存在许多挑战,如细胞来源有限、细胞增殖缓慢等。 方法二:向下分化法 向下分化法是一种常用的方法,可将IPSC分化为RPE细胞。该方法利用特定的培养...
Collectin 11 expression by hypoxia-stressed iPSC-derived RPE cells is linked to complement activationFor decades, complement has been recognized as an effector arm of the immune system that contributes to the destruction of tumor cells. In fact, many therapeutic strategies have been proposed that ...
Methods : iPSC line from a healthy donor (wildtype, WT-iPSC) was genetically modified to generate a CFH-KO-iPSC using CRISPR/Cas9 technology. Both WT- and CFH-KO-iPSC lines were differentiated into RPE, endothelial cells (EC), pericytes, and fibroblasts and validated by immunophenotyping. ...
We aim to use patient-derived iPSC-RPE (induced pluripotent stem cell-derived retinal pigment epithelium) to identify novel disrupted pathways and therapeutic targets. Methods : We generated iPSC-RPE from a CHMS190X patient-derived fibroblast line (n=3), and two wildtype control lines (n=3)....
BVMD; BEST1; calcium-activated chloride channel; Ca2+-activated Cl− channel; RPE; iPSC-RPE; hPSC-RPE; phagocytosis; bestrophinopathy; Best Disease1. Introduction Bestrophinopathies are a group of inherited retinal dystrophies that typically affect the macular region, impairing central vision. ...
RPE dysfunction is the main cause of secondary damage to the photoreceptors. We hereby present RPE implantation into the minipig eye with a 2‐week follow‐up. Methods: Cultured human iPSC‐derived RPEs (iRPE) on nanofibrous membranes, with properties very similar to Bruch's membrane, were ...
CHM/ iPSC-RPE cells demonstrated increased mTORC1 signaling and reduced autophagic flux, consistent with Rab12 dysfunction. Autophagic flux was rescued in CHM/ cells by transduction with gene replacement (ShH10-CMV-CHM) and was reduced in control cells by siRNA knockdown of Ra...
PURPOSE. RPE cell transplantation as a potential treatment for AMD has been extensively investigated; however, in AMD, ultrastructural damage affects both the RPE and its underlying matrix support, the Bruch's membrane (BrM). An RPE monolayer supported by a surrogate scaffold could thus provide a...