Malik, Assessing iPSC reprogramming methods for their suit- ability in translational medicine, J. Cell Biochem. 113 (2012) 3061e3068.Rao MS, Malik N: Assessing iPSC reprogramming methods for their suitability in translational medicine. J Cell Biochem 2012, 113:3061-3068....
Viralreprogramming cells to iPSCs, using lentiviral or retroviral-based transduction methods are the most efficient but have distinct drawbacks for clinical and translational applications Non-viraltransposons technology such asTcBuster™is a next-generation solution that avoids some of the pitfalls of vir...
Other reprogramming methods, such as lentivirus, contain transgenes that can integrate into the host genome, potentially disrupting the genome or causing unpredictable results. As oriP/EBNA1 vectors, these episomal vectors contain 5 reprogramming factors (Oct4, Sox2, Lin28, Klf4, and L-Myc) ...
HighQC™成纤维细胞来源的IPSC货号:ST269价格: ¥询价 规格: 1 vial 数量: - + 在线咨询 产品描述 参考文献 资料下载 相关产品 产品描述/Products Description These pluripotent cells were generated from normal human skin fibroblasts using episomal reprogramming methods. This method allows the transient ...
GMP iPSC Reprogramming kit CTS CytoTune-iPS 2.1 Sendai Reprogramming Kit The first off-the-shelf reprogramming system manufactured in accordance with GMP requirements, offering high-efficiency Sendai delivery of reprogramming factors. Xeno-free workflow—generate ...
With the StemMACS iPSC mRNA Reprogramming Kit, human iPSCs can be generated within 14 days under feeder-free conditions without the need for a conditioned medium or the use of B18R protein. The used mRNA-based approach in contrast to traditional viral- or DNA-based reprogramming methods elimina...
3. Zhang XB: Cellular reprogramming of human peripheral blood cells. Genomics Proteomics Bioinformatics. 2013 Oct;11(5):264-74. 4. Su RJ, Neises A, Zhang XB: Generation of iPS cells from human peripheral blood mononuclear cells using episomal vectors. Methods Mol Biol. 2016;1357:57-69. 5...
GMP iPSC Reprogramming kit CTS CytoTune-iPS 2.1 Sendai Reprogramming Kit The first off-the-shelf reprogramming system manufactured in accordance with GMP requirements, offering high-efficiency Sendai delivery of reprogramming factors. Xeno-free workflow—generate iPSC lines from...
, we demonstrate improved derivation efficiencies of vector-free human iPSCs with an episomal approach. This simplified E8 medium should facilitate both the research use and clinical applications of human ESCs and iPSCs and their derivatives, and should be applicable to other reprogramming methods....
2010年3月23日,BioGatekeeper公司以“Methods for Reprogramming Somatic Cells”为名向美国相关部门申请专利,并获得批准,专利申请人为Rudolf Jaenisch和Konrad Hochedlinger,其美国专利号为NO.7,682,828。 左边为山中伸弥的专利,右边为BioGatekeeper申请的专利 ...