Reprogramming Peripheral Blood Mononuclear Cells (PBMCs) with the CytoTune®-iPS 2.0 Sendai Reprogramming Kit under feeder-free conditions Quicklinks Introduction Ordering Information Materials Needed Guidelines for Reprogramming Preparing Media Preparing MEF dishes...
Reprogramming Peripheral Blood Mononuclear Cells (PBMCs) with the CytoTune®-iPS 2.0 Sendai Reprogramming Kit under feeder-free conditions Quicklinks Introduction Ordering Information Materials Needed Guidelines for Reprogramming Preparing Media Preparing MEF dishes...
An Optimized Protocol for piggyBac-Induced iPSC Generation from hPBMCs by Automatic Electroporationdoi:10.1007/7651_2023_500Thanks to the Yamanaka transcription factors, pluripotency is recovered in the cell culture dish during in vitro cell reprogramming. Induced pluripotent stem cells (iPSCs) have ...
The iPSC clones express proper pluripotentmarkers and differentiate into three lineages. These studiesdemonstrate an easy to use method to generate iPSCs fromperipheral blood and facilitate the transition to high production ...
Reprogramming of Human Peripheral Blood Mononuclear Cell (PBMC) from a Chinese patient suffering Duchenne muscular dystrophy to iPSC line (SDQLCHi007-A) carrying deletion of 49 to 50 exons in the DMD genedoi:10.1016/j.scr.2019.101666Jingyun Guan...
Reprogramming of human peripheral blood mononuclear cell (PBMC) from a patient suffering from hearing loss into iPSC line (SDQLCHi035-A) maintaining compound heterozygous variations in GJB2 gene - ScienceDirectdoi:10.1016/j.scr.2021.102188Xiaomeng Yang a...
Reprogramming to induced pluripotency The iPSCs were generated from PBMCs as previously described11. Briefly, a 10 ml blood aliquot was used for the isolation of PBMCs bydensity gradient centrifugation. The PBMCs were reprogrammed into iPSCs with an integration-freeSendai virusgene delivery method ...
An isogenic control iPSC line, MCRIi028-A-1, was derived from the COL6A1 patient’s PBMCs (https://hpscreg.eu/user/cellline/edit/MCRIi028-A) using a one-step reprogramming and gene editing strategy (Wen et al., 2018). Patient PBMCs were co-transfected with episomal reprogramming vecto...
Type of cell line iPSC Origin Human Additional origin info requiredfor human ESC or iPSC Age:38Sex: femaleEthnicity if known: Chinese Han Cell Source Peripheral blood mononuclear cells (PBMCs) Clonality Clonal Method of reprogramming Sendai Virus Evidence of the reprogramming transgene loss RT-PCR ...
Reprogramming of human peripheral blood mononuclear cells from a patient suffering from recurrent hydatidiform mole to an iPSC line FAHZUi001-A carrying a homozygous p.Gln421Ter mutation in NLRP7 gene Stem Cell Research, Volume 53, 2021, Article 102361 Tingyu Gong,…, Jianhua Qian View PDFShow...