This unit describes differentiation, quality control, culture parameters, and troubleshooting tips to ensure the highest quality and reproducibility of research results. 2019 The Authors. Basic Protocol 1: Differentiation of iPSCs into midbrain‐patterned neural progenitor cells Support Protocol: Quality ...
Find additional tips, troubleshooting help, and resources within ourCell Culture Support Center. No. The Epi5 Episomal iPSC Reprogramming Kit (Cat. No. A15960) was designed to reprogram human cells, not mouse cells. We currently only have data with this kit on reprogramming human cells, which...
Note: If you experience a high number of trypan blue positive/dead cells, please refer to the troubleshooting section, problem 3, for potential solutions. Note: Although described in the context of single cell RNA sequencing workup at an S3 laboratory, the organoid dissociation step of the prot...
Cell culture CRISPR Neuroscience Proteomics Stem Cells Cell Differentiation Mass Spectrometry Before you begin The protocol below describes steps that are specific to the iPSC AST23 cell line, derived from a patient carrying a triplication of the α-synuclein gene, however, the protocol is also suita...
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center. No. The Epi5 Episomal iPSC Reprogramming Kit (Cat. No. A15960) was designed to reprogram human cells, not mouse cells. We currently only have data with this kit on reprogramming human cells, ...
The iPS cells and their derivatives thereof (which derivatives, include without limitation differentiated cells) made by commercial purchasers from this product are for internal research purposes only and may not be (a) used for any commercial purposes, (b) used in or administered into humans, nor...
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center. When generating iPSCs using the Epi5 Episomal iPSC Reprogramming Kit (Cat. No. A15960) cells should be approximately 75-90% confluent on the day of transfection (Day 0). Since over-confluency ...
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center. No. The Epi5 Episomal iPSC Reprogramming Kit (Cat. No. A15960) was designed to reprogram human cells, not mouse cells. We currently only have data with this kit on reprogramming human cells, ...