Cell cultureDifferentiationProgenitorsInduced pluripotent stem (iPS) cells are the product of adult somatic cell reprogramming to an embryonic-like state by inducing a "forced" expression of specific genes. They are similar to natural pluripotent stem cells, such as embryonic stem (ES) cells, in ...
Measured by its ability to support iPS cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is 0.12ug/mL. 推荐产品 相关产品 参考文献 [1]Shankar K, Capitini CM, Saha K. Genome Engineering of Induced Pluripotent Stem Cells to Manufacture Natural ...
[Methods in Molecular Biology] Pluripotent Stem Cells Volume 997 || A Review of the Methods for Human iPSC Derivation U Lakshmipathy,MC Vemuri 被引量: 0发表: 2013年 Chemically defined conditions for human iPSC derivation and culture We reexamine the individual components for human ES and iPS ...
参考资料: [1] How Do Cells Cultured in 2D and 3D Differ? A Dimension Shift [2] Ackermann, M., Rafiei Hashtchin, A., Manstein, F. et al. Continuous human iPSC-macrophage mass production by suspension culture in stirred ...
cells was carried out on 96-Transwell inserts at ALI to resemble the physiological environment of the mature human alveolar epithelium. The here described differentiation protocol (illustrated in Fig.1a), which focused on culture conditions and suitability in upscaled applications, incorporates various ...
The calculated area of stained cells is shown in the bar graph. n = 6. D Effect of FGF2 on HUVEC proliferation. 1.5 or 10 ng/mL FGF2 was added to the culture medium, and the cell number was counted after 72 h of incubation. n = 6. E FGFR-inhibition assay. The FGFR ...
针对人诱导多能干细胞(induced pluripotent stem cells,iPSC)科学研究与应用需求不断增长、市场上并没有一款iPSC 3D培养专用设备的困境,睿生生物科研团队经过不断攻关,成功推出全国首款DASEA®Regenbio全自动3D iPSC培养系统,为iPSC的规模化自动化制备铺平道路!
针对人诱导多能干细胞(induced pluripotent stem cells,iPSC)科学研究与应用需求不断增长、市场上并没有一款iPSC 3D培养专用设备的困境,睿生生物科研团队经过不断攻关,成功推出全国首款DASEA®Regenbio全自动3D iPSC培养系统,为iPSC的规模化自动化制备铺平道路!
诱导多能干细胞 (iPSC) 是利用各种方法从成纤维细胞等成体细胞类型中衍生而来的。iPSC 通常维持在一层失活的鼠胚胎成纤维细胞 (MEF) 饲养层细胞上。细胞可以在这些条件下维持数代,且不影响细胞的增殖或多能性和分化潜能。本实验方案描述了如何在饲养层细胞上培养成熟的 iPSC。
In vitro culture of hematopoietic stem and progenitor cells (HPCs) is supported by a suitable cellular microenvironment, such as mesenchymal stromal cells (MSCs)—but MSCs are heterogeneous and poorly defined. In this study, we analyzed whether MSCs derived from induced pluripotent stem cells (iPS...