此外还有CompPASS (Comparative Proteomic Analysis Software Suite),MiST (mass spectrometry interaction statistics)和SAINT (Significance Analysis of INTeractome)评分算法,基于蛋白在各个样品中的定量数据对蛋白进行评分,将评分高于特定阈值的作为互作蛋白,但这些评分算法往往使用蛋白鉴定到的谱图数作为定量数据,随着非标记...
此外还有CompPASS (Comparative Proteomic Analysis Software Suite),MiST (mass spectrometry interaction statistics)和SAINT (Significance Analysis of INTeractome)评分算法,基于蛋白在各个样品中的定量数据对蛋白进行评分,将评分高于特定阈值的作为互作蛋白,但这些评分算法往往使用蛋白鉴定到的谱图数作为定量数据,随着非标记...
此外还有CompPASS (Comparative Proteomic Analysis Software Suite),MiST (mass spectrometry interaction statistics)和SAINT (Significance Analysis of INTeractome)评分算法,基于蛋白在各个样品中的定量数据对蛋白进行评分,将评分高于特定阈值的作为互作蛋白,但这些评分算法往往使用蛋白鉴定到的谱图数作为定量数据,随着非标记...
建议通过向洗涤缓冲液中加入去垢剂优化洗涤缓冲液。参考文献 Dragana L ,L. K K ,C.-H. H L, et al. An optimized co-immunoprecipitation protocol for the analysis of endogenous protein-protein interactions in cell lines using mass spectrometry[J].STAR Protocols,2022,3(1):101234-101234.
参考文献 Dragana L ,L. K K ,C.-H. H L, et al. An optimized co-immunoprecipitation protocol for the analysis of endogenous protein-protein interactions in cell lines using mass spectrometry[J].STAR Protocols,2022,3(1):101234-101234.
免疫沉淀技术是一种基于抗体和抗原特异性结合的原理研究蛋白质与蛋白质相互作用的方法,将细胞裂解液或组织提取液等蛋白混合体系与结合有目的蛋白的磁珠进行孵化,混合体系中能与目的蛋白相互作用的蛋白质通过特异性结合而与其他蛋白组分分离开来,再通过离心或洗脱去掉没有与目的蛋白结合的杂蛋白,从而获得与目的蛋白质相互...
MAIER V K, FEENEY C M, TAYLOR J E, et al. 2015. Functional Proteomic Analysis of Repressive Histone Methyltransferase Complexes Reveals ZNF518B as a G9A Regulator. Mol Cell Proteomics [J], 14: 1435-1446. POURFARZAD F, AGHAJANIREFAH A, DE BOER E, et al. 2013. Locus-specific proteomic...
MAIER V K, FEENEY C M, TAYLOR J E, et al. 2015. Functional Proteomic Analysis of Repressive Histone Methyltransferase Complexes Reveals ZNF518B as a G9A Regulator. Mol Cell Proteomics [J], 14: 1435-1446. POURFARZAD F, AGHAJANIREFAH A, DE BOER E, et al. 2013. Locus-specific proteomic...
1. IWASAKI M, MINDER P, CAì Y, et al. 2018. Interactome analysis of the lymphocytic choriomeningitis virus nucleoprotein in infected cells reveals ATPase Na+/K+ transporting subunit Alpha 1 and prohibitin as host-cell factors involved in the life cycle of mammarenaviruses. PLoS Pathog [J],...
4. Data Analysis. Identify enriched proteins in IP sample compared to control. Validate interactions using orthogonal methods (e.g., co-immunoprecipitation, crosslinking). Advantages of IP-MS: Detects direct and indirect protein interactions. Provides quantitative information about protein abundance. Can...