In: PCR Technology: Principles and Applications, ed. H.A. Erlich, New York: Stockton Press.Ochman H, Ajioka JW, Garza D, Hartl DL (1990) Inverse polymerase chain reaction. Biotechnology (NY) 8:759-760Ochman, H., Ajioka, J. W., Garza, D., and Hartl, D. L., Inverse polymerase ...
https://geneticeducation.co.in/inverse-pcr-principle-procedure-protocol-and-applications/#Protocol_for_inverse_PCR 网址:里面设计了IPCR的相关内容包括,酶切酶连,限制性位点选择,Procedure等。 Inverse polymerase chain reaction(Inverse PCR) is a variant of the polymerase chain reaction that is used to am...
6) PCR-reverse hybridizaion 聚合酶链反应-反向杂交 补充资料:聚合酶链反应 聚合酶链反应 polymerase chain reaction,PCR 是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA)片断大量增扩,达到用常规方法就可以检测的水平。临床...
Objective Inverse polymerase chain reaction(IPCR) was used to detect the integration of hepatitis B virus (HBV) DNA in HBV related human hepatocellular carcinomas (HCC). Methods IPCR was based on the simple procedures of digestion of DNA with restriction enzymes and circularization of cleavage pro...
Polymerase Chain ReactionA PCR-based assay was developed for typing L. interrogans sensu lato serovars. The assay is designed to exploit the presence of ... RL Zuerner,D Alt,CA Bolin - 《Journal of Clinical Microbiology》 被引量: 187发表: 1995年 Detection and identification of Leptospira int...
The circular DNA is used as a template for polymerase chain reaction (PCR), using primers designed against known sequences. The resulting PCR product contains an unknown segment that is flanked by known sequences.This is a preview of subscription content, log in via an institution to check ...
The new method, termed enzymatic inverse polymerase chain reaction (EIPCR), u... WPC Stemmer,SK Morris - 《Biotechniques》 被引量: 224发表: 1992年 Site-Directed Mutagenesis In Vitro by Megaprimer PCR Among the various mutagenesis procedures based on polymerase chain reaction (PCR), the "mega...
L. (1988) Genetic applications of an inverse polymerase chain reaction. Genetics 120, 621–625. PubMed CAS Google Scholar Triglia T., Peterson M. G., and Kemp D. J. (1988) A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences. ...
网络释义multiplicative inverse 乘法逆元; 乘法逆运算; 乘法逆; 乘法逆元素; multiplicative inverse 乘法逆元; 乘法逆运算; 乘法逆; 乘法逆元素; INVERSE PCR Inverse Polymerase Chain Reaction 反向聚合酶链反 应; inverse Matrix matrix inverse 矩阵求逆; completely multiplicative 完全积性的;...
Hemsley A, Arnheim N, Toney MD, Cortopassi G, Galas DJ (1989) A simple method for site-directed mutagenesis using the polymerase chain reaction. Nucleic Acids Res 17(16):6545–6551 Article CAS Google Scholar Qi D, Scholthof KB (2008) A one-step PCR-based method for rapid and efficient...