The reaction in which multiple copies of the gene (or DNA) of interest is synthesised invitro using two sets of primers & enzyme DNA polymerase is known as polymerase chain Reaction. Enzyme used is thermostable DNA polymerase isolated from the bacterium, Thermus aquaticus. Show More ...
Use of urine polymerase chain reaction to define the prevalence and clinical presentation of Trichomonas vaginalis in men attending an STD clinic. Sex Transm Infect 2003; 79: 151-153.Wendel KA et al. Use of urine polymerase chain reaction to define the prevalence and clinical presentation of ...
In each case, polymerase chain reaction (PCR) was performed for Line1 79 bp, Line1 300 bp, Alu 115 bp, Alu 247 bp, and mitochondrial primers. In addition, carcinoembryonic antigen (CEA) was measured by ELISA. Each marker was analysed between normal, polyp, and cancer populations, and ...
1–3 μg DNA was sheared to 100–1,000 bp using a Covaris E210 or LE220 and size selected (350–450 bp) using magnetic beads (Ampure XP). Sheared DNA was subjected to Illumina paired-end DNA library preparation and PCR-amplified for six cycles. Amplified...
Polymerase Chain Reactions (PCR) were performed using the Q5 high fidelity DNA polymerase (New England BioLabs) for routine cloning or Pfu Turbo (Agilent) for high fidelity and quick-change mutagenesis. Custom oligonucleotides, listed in Table S2, were synthesized by IDT Integrated DNA Technologies ...
We are living in a dynamic world where testing standards and technologies are constantly changing. The most accurate tests available are polymerase chain reaction (PCR) tests, which can take up to several days to get a result. Rapid tests, on the other hand, are not as accurate, but they ...
we enrolled 171 patients affected by occasional mild, moderate or severe conjunctivitis in a three-arm prospective open study, using traditional analysis such as Immune Fluorescent Assay and Enzyme鈥揕inked Fluorescent Assay (IFA and ELFA) and molecular analysis with Polymerase Chain Reaction (PCR) pr...
After ligation of genomic fragments with a compatible DNA linker, linker-mediated polymerase chain reaction (LM-PCR) allows the amplification of the virus-host genome junctions. Massive sequencing of the amplicons is used to define the mLV integration profile genome-wide. Finally, clusters of ...
PCR polymerase chain reaction PAGE polyacrylamide gel electrophoresis GFP green fluorescent protein Epsin (epsin 1) is a recently characterized protein with a putative role in clathrin-mediated endocytosis. Its COOH-terminal region, which contains three repeats of the EH domain binding consensus NPF, ...
PCR: Polymerase chain reaction PDD: Pervasive developmental disorder PDD-NOS: Pervasive developmental disorder-not otherwise specified RasGAP: Ras GTPase activating protein RCPM: Raven’s colored progressive matrices SCZ: Schizophrenia SD: Standard deviation SNRPN: Small nuclear ribonucleoprotein ...