Despite the immunologic functions of T-cell receptor signaling molecules being extensively investigated, their potential as immunohistochemical markers has been poorly explored. With this background, we evaluated the expression of 5 intracellular proteins-GADS, D0K2, SKAP55, ITK, and PKCalpha-involved ...
Empty CellTotal waterIntracellular fluidECFInterstitial fluidIntravascular fluid Man (adult)655015105 Woman (adult)604515105 Infant754827225 Values are expressed as percent of total body weight. ECF, Extracellular fluid. An additional compartment is represented by thetranscellularspace, which comprises the liq...
Thereafter, cells were stained with fluorescence- labeled antibodies specific for intracellular cytokines (IL-2, IL-4, IFN-gamma, and tumor necrosis factor (TNF)-alpha and cell surface markers CD4 and CD8, and were subjected to flow-cytometric analysis. In bronchoalveolar lavage fluid (BALF), ...
Enzyme markers assured adequate separation of the organelles. Control transport assays were performed at 4° C without ATP. Results: Transport of 3H-TG from the ER to the Golgi was linear for 15 min. Golgi from donor rats accepted 3H-TG from the ER in increasing amounts up to 100 μg ...
Allogeneic hematopoietic cell transplantation for adults with acute myeloid leukemia: myths, controversies, and unknowns Progress in the last decade has improved the understanding of leukemia biology. Molecular markers in combinations with cytogenetics have improved the risk ... V Gupta,MS Tallman,DJ ...
IL-15 is produced by a subset of human melanomas, and is involved in the regulation of markers of melanoma progression through juxtacrine loops. IL-15 is a novel cytokine active through the IL-2R/betagamma. Since several human melanoma cell lines display functional IL-2Rs, we studied the ...
To examine whether ASIC1a Cell Death and Differentiation 1364 Mitochondrial ASIC1a regulates oxidative cell death T-L Xu et al Figure 4 ASIC1a is expressed in mitochondria of mouse cortical neurons. (a) Presence of ASIC1a and organelle markers (PDI for ER, glyceraldehyde-3-phosphate dehydrogenase...
(MDSCs) in aF. nucleatum-inoculated CRC mouse model was accompanied by T-cell suppression and increased expression of immunosuppressive molecules such as CTLA4 and arginase-117,154. Recruitment of myeloid cells by intratumoral bacteria may also contribute to inflammation via activation of the Janus ...
Immunohistochemical analysis of paraffin-embedded Jurkat (left) and 293 (right) cell pellets using CD45 (Intracellular Domain) (D9M8I) XP® Rabbit mAb. Confocal immunofluorescent analysis of Jurkat (left) and 293T (right) cells using CD45 (Intracellular Domain) (D9M8I) XP® Rabbit mAb (gr...
Critical cancer pathways often cannot be targeted because of limited efficiency crossing cell membranes. Here we report the development of a Salmonella-based intracellular delivery system to address this challenge. We engineer genetic circuits that (1) a