High Sensitivity Intact Mass Analysis of Antibodies (IgG1) Using ionKey/MSINTRODUCTIONLC-MS has emerged as a powerful and robust tool for the characterization of intact proteins. This approach has been widely applied to heterogeneous therapeuticmonoclonal antibodies, antibody drug conjugates, bispecific ...
Intactmassanalysisofmonoclonalantibodiesbycapillary electrophoresis—Massspectrometry MeiHan ∗ ,BrookeM.Rock,JoshT.Pearson,DanA.Rock PharmacokineticsandDrugMetabolism,AmgenInc.,1120VeteransBoulevard,SouthSanFrancisco,CA,USA articleinfo Articlehistory:
Herein, we report implementation of this technology for coupling CE to time-of-flight mass spectrometer for intact mass analysis of antibodies. Abstract Characterization of monoclonal antibody (mAb) therapeutics by intact mass analysis provides important information on sequence integrity and post-...
Intact mass analysis of monoclonal antibodies by capillary electrophoresis-Mass spectrometry. Characterization of monoclonal antibody (mAb) therapeutics by intact mass analysis provides important information on sequence integrity and post-translatio... H Mei,BM Rock,JT Pearson,... - 《J Chromatogr B ...
Poster: Intact Mass Analysis of Lysine-Linked Antibody-Drug Conjugates using Microfluidic Chip Electrophoresis and Size Exclusion Chromatography Coupled to an Orbitrap Mass Spectrometer Support LC-MS Instrument Support Center LC-MS Software Support Center ...
Poster: Intact Mass Analysis of Lysine-Linked Antibody-Drug Conjugates using Microfluidic Chip Electrophoresis and Size Exclusion Chromatography Coupled to an Orbitrap Mass Spectrometer Support LC-MS Instrument Support Center LC-MS Software Support Center ...
Recently, we introduced a new approach for the analysis of the intact proteins based on ion mobility-mass spectrometry (IM-MS). Our protocol involves the collision induced unfolding (CIU) of intact antibodies, where collisional heating in the gas-phase is used to generate unfolded antibody forms...
In an earlier installment, we discussed the role of liquid chromatography and mass spectrometry in MAMs. In this article, we focus on intact mass analysis-based multi-attribute methods (MAMs), a suitable alternative that can complement standard MAMs or be used when there is a need for rapid ...
Intact Mass Poster Note Capillary Electrophoresis – Mass Spectrometry for Intact Mass Analysis of Antibodies and Antibody-Drug-Conjugates Life Science Mass Spec Intact Mass Case Study Simple, Robust, High Quality Intact Mass Analysis—Biosimilars L...
Intact mass analysis and native mass spectrometry Biopharmaceutical characterization is required throughout all stages of drug development and manufacturing. During drug development, liquid chromatography–mass spectrometry(LC-MS) approaches are used to ...