We began the quest by examining the role of TonEBP in adipogenesis and adipocyte inflammation. Here we report that TonEBP suppresses the epigenomic transition of the peroxisome proliferator-activated receptor gamma 2(PPARγ 2) promoter and adipogenesis while promoting adipocyte inflammation. School ...
To identify the DNA-binding proteins in an antibody (Ab) disruption assay, polyclonal antibodies specific to NRF-1 or normal rabbit control IgG were added to the binding reactions and incubated for 20 min at room temperature before probes were added (lanes 9 and 10). B, the association of ...
Interestingly, it has been observed that healthy human centenarians likely have IGF-1 receptor genetic variants associated with reduced function, an intriguing observation that correlates with our findings. As ILS pathway is part of the nutrient signalling pathway, it is also (negatively) controlled ...
Among the various types of inflammasomes, the nod-like receptor protein 3 (NLRP3) inflammasome has been extensively studied in adipose tissue. The Nlrp3, Casp1 and Il1b knockout mice were protected from high-fat diet-induced adipose insulin resistance [15, 16, 17, 18], but it is unclear...
Through interaction between neural and direct receptor-dependent mechanisms, GLP-1 regulates multiple metabolic and car- diovascular functions (5,8). Moreover, GLP-1 is produced by neurons in the brainstem (9,10) and transported to From 1INSERM U1048, Institute of Metabolic and Cardiovascular ...
elegans homologue of the insulin-like receptor (IGFR), a transmembrane receptor that negatively regulates DAF-16 and HSF-1 (ref. 69). Loss-of-function mutations affecting DAF-2 have been shown to increase longevity and resistance to heat stress68. Interestingly, most of the positive lifespan ...
In addition to monitoring changes in the expression of these adipogenic proteins, changes in adipokine secretion following treatment with AZD3965 were also assessed utilizing an adipokine antibody microarray. Conditioned media collected from adipocytes that had been treated with or without AZD3965 for 72...