To determine expression levels of serum leptin, liver leptin receptor, PTP-1B and PPARα/γ, ELISA was performed using the protein-specific monoclonal antibody. Proteins in the samples were diluted to reach the concentration within the range of standard curve. Other procedures followed instructions ...
(18), in a cholesterol gallstone mouse model, reported that Cyp7b1 mRNA levels were markedly suppressed in liver selective insulin receptor knockout mice. However, the potential importance of regulation of CYP7B1 by insulin has never been truly addressed in the context of NAFLD and NASH. NAFLD...
Elhanany E, Kanety H, Zick Y (1997) A molecular basis for insulin resistance: elevated serine/threonine phos‑ phorylation of irs-1 and irs-2 inhibits their binding to the juxtamembrane region of the insulin receptor and impairs their ability to undergo insulin- induced tyrosine phosphorylatio...
We began the quest by examining the role of TonEBP in adipogenesis and adipocyte inflammation. Here we report that TonEBP suppresses the epigenomic transition of the peroxisome proliferator-activated receptor gamma 2(PPARγ2) promoter and adipogenesis while promoting adipocyte inflammation....
Interestingly, it has been observed that healthy human centenarians likely have IGF-1 receptor genetic variants associated with reduced function, an intriguing observation that correlates with our findings. As ILS pathway is part of the nutrient signalling pathway, it is also (negatively) controlled ...
Through interaction between neural and direct receptor-dependent mechanisms, GLP-1 regulates multiple metabolic and car- diovascular functions (5,8). Moreover, GLP-1 is produced by neurons in the brainstem (9,10) and transported to From 1INSERM U1048, Institute of Metabolic and Cardiovascular ...
The blots were subsequently incubated with peroxidase-conjugated secondary antibody for 1 h, and processed for enhanced chemiluminescence to visualize the immunoreactive bands. Band intensities were quantized by optical densitometry (Scion Image-Release Beta 3b, NIH, USA) of the developed autoradiography....
Among the various types of inflammasomes, the nod-like receptor protein 3 (NLRP3) inflammasome has been extensively studied in adipose tissue. The Nlrp3, Casp1 and Il1b knockout mice were protected from high-fat diet-induced adipose insulin resistance [15, 16, 17, 18], but it is unclear...
(D) Photographs showing increased liver size in CerS2 heterozygous mice (a), CerS2 heterozygous and wild-type mouse liver histological sections stained with hematoxylin and eosin (b) and oil red O (c), and immunohistochemistry using an antibody against the F4/80 antigen found in murine macropha...
Baserga, Renato, “The Insulin-like Growth Factor I Receptor: A Key to Tumor Growth&quest ” Cancer Research (1995) 55:249-252. Carter et al., “The Prostate: An Increasing Medical Problem,” Prostate (1990) 16:39-48. Catalona et al., “Prostate Cancer Detection in Men With Serum ...