Immuno-enzymatic test for anti-insulin antibodiesThe suit is made of a piece of material which can cover the body several times and provided with various fastening means which can be joined in many directionsTRIOLO GIOVANNI
G. Increased feeding in response to bilateral injection of insulin antibodies in the VMH. Physiol. Behav. 19, 309–313 (1977). Article CAS PubMed Google Scholar Woods, S. C. & Porte, D. Jr. The role of insulin as a satiety factor in the central nervous system. Adv. Metab. Disord...
He was allergic to insulin, as shown by a raised eosinophil count, a markedly increased IgE concentration, and antibodies to human, bovine, and porcine insulins in the RAST test. He was not allergic to needles or additives. With subcutaneous bolus doses of insulin lispro and Humulin he ...
The input and precipitated protein samples were subjected to western blot analysis with corresponding antibodies as stated. Western blots of the elution of the control group were used to control for any non-specific binding of the beads. The specific bands from SDS–PAGE were cut and digested, ...
All primary antibodies had been used in studies published in multiple journals. As previously described (18), staining was performed using the streptavidin-biotin and peroxidase method, using the manufacturer's protocol (DAKO Catalyzed Signal Amplification System, HRP, DAKO Cytomation, Carpinteria, ...
enrolled relatives who were positive for islet cell antibodies by immunofluorescence and positive for insulin autoantibodies by radioimmunoassay, had first-phase insulin release on intravenous glucose tolerance test higher than threshold (defined below), and had a normal oral glucose tolerance test result....
Summary. Nine insulin-dependent diabetics with undetectable plasma C-peptide (-1) and without insulin antibodies (insulin binding to IgG-1) received subcut... E Fernqvist,B Linde,J Östman,... - 《Clinical Physiology》 被引量: 57发表: 1986年 加载更多来源...
The cells were incubated with diluted anti-GFP antibodies (1:500, homemade81) in 3% BSA in 0.1% PBST overnight at 4 °C. After wash, the cells were incubated with fluorescent secondary antibodies (1:200, Alexa fluor 488 goat anti-rabbit IgG (H + L) antibody, Cat. #A11008, ...
It was reported previously that ~5% of STC-1 cells produce GLP-1, whereas no immunoreactivity was detected with insulin antibodies [12]. Because the STC-1 cell line is a suitable source of intestinal cells, it was used for isolation of the L-cells. The STC-1 cells were transfected with...
incubated with protein A Dynabeads (cat. no. 10008D; Thermo Fisher Scientific) pre-bound with normal rabbit IgG (0.5 μg/IP; cat. no. 10500 C; Invitrogen) or normal mouse IgG (0.5 μg/IP; cat. no. 10400 C; Invitrogen), and the indicated antibodies (0.5 μg/IP)....