The wt. of the dried precipitate is converted to biological activity through a factor of 25[mu]./mg. Comparison of biol. assays with duplicate fibril precipitation assays on 64 unselected still concentrates indicated the assay in vitro to have a precision error within [plus or minus] 5%. ...
somatomedin C also caused inhibition of glycerol releasein vitroin epinephrine-stimulated rat epididymal fat tissue (Underwoodet al.,1972). In this system 1 unit of somatomedin was equivalent to 120μU of porcine insulin. Somatomedin C also suppressed the adenylate cyclase activity of fat cell ...
The present invention includes an assay system which may be prepared in the form of a test kit for the quantitative analysis of the extent of messenger material or enzyme activity. The system or test kit may comprise a labeled component prepared by one of the radioactive and/or enzymatic tech...
To address this possibility, direct competition between Aβ and Drosophila ILPs was tested by an in vitro degradation assay wherein Escherichia coli-expressed Drosophila IDE and full-length ILPs proteins were co-incubated. These purified ILPs were capable of functionally inducing insulin signaling in...
Six different in vitro-translated GST-ERRα proteins were tested in a kinase assay, validating that ERRα can be directly phosphorylated by GSK3β (Fig. 3e). Mutation of any of these three serine residues to alanine markedly decreased GSK3β-mediated ERRα phosphorylation (Fig. 3e). ...
: Assay of insulin-like activity by the isolated fat cell method. I. Factors influencing the response to crystalline insulin. Diabetologia 3 , 382 (1967).Gliemann, J., Sorensen, I{.I-I.: Assay of insulin-like activity by the isolated fat cell method. IV. The bio- logical activity of...
Body weight, food intake, visceral fat weight, uterine weight, serum lipid profile, glucose tolerance, insulin action on skeletal muscle glucose transport activity, and GLUT-4 protein expression were determined. Results Prolonged ovariectomy resulted in dyslipidemia, impaired glucose tolerance and insulin...
In vitro-deranged intestinal immune response to gliadin in type 1 diabetes. Diabetes. 2004;53(7):1680-168315220190PubMedGoogle ScholarCrossref 28. Mojibian M, Chakir H, Lefebvre DE, et al. Diabetes-specific HLA-DR–restricted proinflammatory T-cell response to wheat polypeptides in tissue ...
in PGH levels, in concert with cortisol, progesterone, TNF-α, and decreased adiponectin levels, greatly augments maternal insulin resistance. PGH levels in pregnant women correlate with the birth weight of their offspring. The in vitro PGH secretion in a human trophoblast model was found to be ...
Each assay was done in triplicate and expressed as the mean ± S.D. Electrophoretic Mobility Shift Assay (EMSA)—FoxO1 and FoxA1 were synthesized in vitro using the transcription/translation (TNT) system programmed with the FoxO1 and FoxA1 expression plasmids according to the manufacturer's ...