We have prepared inside-out membrane vesicles from rabbit neutrophils. These vesicles are capable of actively transporting calcium. This active transport requires the presence of Mg ATP. Other nucleotides such as UTP, GTP, CTP, AMP, and ADP are not able to stimulate the active uptake of calcium...
Active Na transport into inside-out vesicles (IOVs) is promoted by addition of the substrates for GAPDH and PGK, without added ATP. The GAPDH鈥揚GK complex synthesizes ATP that remains associated with the red cell membrane. The membrane-associated ATP must be compartmentalized because it is ...
Inside-out membrane vesicles capable in the presence of MgATP of accumulating calcium and containing Ca2+-ATPase activity have been prepared from rabbit neutrophils. The transport system has a Km for Ca2+ of 2.8 +/- 0.3 microM and a Vmax... M Volpi,PH Naccache,RI Sha'Afi - 《Journal of...
All-or-none response of the Ca2+-dependent K+ channel in inside-out vesicles. García-Sancho,Sanchez,Herreros - 《Nature》 被引量: 56发表: 1982年 Reconstitution of periplasmic transport in inside-out membrane vesicles. Energization by ATP. The periplasmic histidine permease of Salmonella ...
9 RegisterLog in Sign up with one click: Facebook Twitter Google Share on Facebook (redirected frominside-outly) Thesaurus ThesaurusAntonymsRelated WordsSynonymsLegend: Switch tonew thesaurus Adj.1.inside-out- with the inside surface on the outside ...
The two surfaces of a membrane can be compared by isolating sealed vesicles which bare one side or the other to impermeable probe molecules. For this purpose, inside-out (IO) and right-side-out (RO) vesicles have been generated from human red blood cell membrane ghosts and partially resolved...
Transport of Ca 2+ by inside-out vesicles requires both Mg 2+ and ATP and can be linear over 16 min at 37°C. This basal rate of transport may be doubled however by an activator found in membrane-free erythrocyte hemolysate. This activator is probably the same protein(s) which has be...
1) inside out vesicles 翻转膜微囊2) everted-gut sacs 翻转肠囊 1. The isolated mucosa experiment,the everted-gut sacs experiment and the Caco-2 cell experiment were used to investigate the paracellular transport of indirubin. 方法:采用高效液相色谱法测定靛玉红的含量;通过缚管翻转肠囊实验、...
Protein biocargo of citrus fruit-derived vesicles reveals heterogeneous transport and extracellular vesicles populations Cellular vesicles are membrane-enclosed organelles that transport material inside and outside the cell. Plant-derived vesicles are receiving increasing att... P Gabriella,T Lilla,A Alfredo...
Human, rat, mouse and dog MRP2 inside out vesicles were prepared from Sf9 insect cells expressing the corresponding transporters. They were supplied by Genomembrane. MRP2-mediated and ATP-dependent uptake of CDCF (5(and6)carboxy2', 7'dichlorofluorescein), a well-known substrate of MRP2, was ...