We have prepared inside-out membrane vesicles from rabbit neutrophils. These vesicles are capable of actively transporting calcium. This active transport requires the presence of Mg ATP. Other nucleotides such as UTP, GTP, CTP, AMP, and ADP are not able to stimulate the active uptake of calcium...
(1988) Inside-out plant plasma membrane vesicles of high purity obtained by aqueous two-phase partitioning. FEBS Lett. 229: 289-292.Larsson C, Widell S, Sommarin M (1988) Inside-out plant plasma membrane vesicles of high purity obtained by aqueous two-phase partitioning. FEBS Lett. 229: ...
Define inside trim. inside trim synonyms, inside trim pronunciation, inside trim translation, English dictionary definition of inside trim. abbr. insulin shock therapy American Heritage® Dictionary of the English Language, Fifth Edition. Copyright
Adj.1.inside-out- with the inside surface on the outside wrong-side-out turned- moved around an axis or center Based on WordNet 3.0, Farlex clipart collection. © 2003-2012 Princeton University, Farlex Inc. Want to thank TFD for its existence?Tell a friend about us, add a link to ...
Inside-out membrane vesicles capable in the presence of MgATP of accumulating calcium and containing Ca2+-ATPase activity have been prepared from rabbit neutrophils. The transport system has a Km for Ca2+ of 2.8 +/- 0.3 microM and a Vmax... M Volpi,PH Naccache,RI Sha'Afi - 《Journal of...
All-or-none response of the Ca2+-dependent K+ channel in inside-out vesicles. García-Sancho,Sanchez,Herreros - 《Nature》 被引量: 56发表: 1982年 Reconstitution of periplasmic transport in inside-out membrane vesicles. Energization by ATP. The periplasmic histidine permease of Salmonella ...
Plasma membrane preparations of high purity (about 95%) are easily obtained by partitioning in aqueous polymer two-phase systems. These preparations, however, mainly contain sealed right-side-out (apoplastic side out) vesicles. Part of these vesicles have been turned inside-out by freezing and tha...
Transport of Ca 2+ by inside-out vesicles requires both Mg 2+ and ATP and can be linear over 16 min at 37°C. This basal rate of transport may be doubled however by an activator found in membrane-free erythrocyte hemolysate. This activator is probably the same protein(s) which has be...
Preparation of impermeable inside-out and right-side-out vesicles from erythrocyte ... JA Kant,TL Steck - 《Nature New Biology》 被引量: 171发表: 1974年 Sugar transport by the bacterial phosphotransferase system. Preparation and characterization of membrane vesicles from mutant and wild type ...
The current model for EV biogenesis dictates that cytosolic proteins remain in the lumen and cell surface proteins reside on the outside of vesicles. This is consistent with the traditional protein trafficking pathway, where proteins destined for the plasma membrane contain a signal sequence targeting...