miR-1254 could suppress GC cell proliferation, whereas inhibition of miR-1254 promoted the effects (Fig.2b). We also used the EDU incorporation assay to examine the effect of miR-1254 on proliferation. As shown in Fig.2c, MGC803 cells transfected with the miR-1254 mimics revealed a remarkab...
evidenced that miR-196 overexpression decreased the proliferation in K562 cells [38]. Furthermore, miR-196b overexpression significantly reduced cell viability, clonogenicity, proliferation and invasion in cervical cancer [32]. In parallel to miR-196b overexpression effects, we show that the IGF2BP1...
Fig. 1. Metformin inhibits cell proliferation in SKM-1 cells. A. SKM-1 cells were treated with metformin (0 mM, 1 mM, 5 mM, 10 mM, 15 mM or 20 mM) for 24, 48 and 72 h, cell proliferation was detected by CCK8 assay. B. SKM-1 cells were treated with metformin (0 mM, 1 ...
Hippo signaling pathway was previously known to control organ size and growth, and accumulating evidence shows that this pathway acts a pivotal role in the regulation of cell proliferation, metastasis and oncogenesis [3,4,5,6]. Large tumor suppressor kinase 1 (LATS1) as a core member of this...
This effect is dependent on a stress-induced rise in adrenal hormones; exposure to fox odor resulted in an increase in circulating corticosterone levels and prevention of this increase (by means of adrenalectomy plus low-dose corticosterone replacement) eliminated the suppression of cell prolife 展开...
Results with P < 0.05, P < 0.01 and P < 0.05 were considered as statistical significance; ns means P > 0.05. 3. Results 3.1. HGP inhibited cell proliferation on HepG2 cells in vitro and suppressed tumor growth in vivo We initially elucidated whether HGP could inhibit ...
proliferation, and cell survival [61]. On the other hand, GSK3β relays a signal transduction cascade involved in cellular processes like gene transcription, cell proliferation, and apoptosis [62]. This protein can be phosphorylated on different serine residues by other kinases including AKT [63]...
Using CCK-8 and colony-formation assay, we found that cell proliferation was significantly reduced. Upon co-transfection of FUT4 cDNA and miR-200b mimics or FUT4 cDNA transfection alone, the proliferation rate was restored or increased significantly (Figures 4a and c, 5a and c). Additionally,...
plantarum strains and (B) the modified culture media (cMC) incubated with HT-29 for 24 and 48 h and evaluated by means of SRB assay. (A) BLP (butyrate-producing strain S2T10D) and LP (non- producing strain O2T60C) were tested: viable bacteria (Vb); bacterial cell-free supernatants...
Metformin (Met) decreased the proliferation, migration, and invasion of HT29, HCT116 and HCT116 p53−/− cells. (a) Cell proliferation was evaluated in vitro by means of BrdU incorporation in the absence (Ctrl) or presence of 5 mM Met after 24, 48 and 72 hours’ treatment. The...