which could have provided more detailed insights into the antibody-HA interaction and affinity, we successfully mapped the binding area of E10 as a conserved β-sheet on the HA surface. Furthermore, while
which could have provided more detailed insights into the antibody-HA interaction and affinity, we successfully mapped the binding area of E10 as a conserved β-sheet on the HA surface. Furthermore, while
260 Bio Thermo spectrophotometer by using cells of 1 cm path length. UV-vis absorption spectra of19and23were registered using a ca. 10−5M solution in methanol. Each metal/ligand system was studied by titrating a 2.8 ml sample of the ligand solution with a methanolic solution of Mg(CH3CO...
J Vis Exp (2014), Article 51875 10.3791/51875 Google Scholar 16. S Hosseini, E Wilk, K Michaelsen-Preusse, I Gerhauser, W Baumgärtner, R Geffers, K Schughart, M Korte Long-term neuroinflammation induced by influenza A virus infection and the impact on hippocampal neuron morphology and ...
Following purification, nanoparticle quality and concentration was first measured by UV-vis spectroscopy. Nanoparticle polydispersity and purity was then assessed using SDS-PAGE, DLS, and nsEM. Finally, endotoxin levels were measured using the LAL assay, with all immunogens used in animal studies cont...
J Vis Exp (2014), Article 51875 10.3791/51875 Google Scholar 16. S Hosseini, E Wilk, K Michaelsen-Preusse, I Gerhauser, W Baumgärtner, R Geffers, K Schughart, M Korte Long-term neuroinflammation induced by influenza A virus infection and the impact on hippocampal neuron morphology and ...