Virologic endpoints for both assays included virus AUC, peak viral load, and duration of viral shedding in subjects treated with VIS410. For biological secondary endpoints, serum levels of cytokines and chemokines (TNFα, IFNγ, IL-6, IL-8, and IL-10) were measured by ELISA and serum HAI...
The High-Performance Liquid Chromatography (HPLC) system, Thermo Dionex UltiMate 3000, is equipped with a binary pump, an auto-sampler, a column oven, and a diode array UV/VIS detector (Thermo Fisher Scientific, San Jose, CA, USA). HPLC-grade acetonitrile and methanol were purchased from Fis...
doi:10.1186/s12879-023-08541-0Carregaro, Rodrigo LuizRoscani, Alessandra N. C. P.Raimundo, Augusto Cesar SousaFerreira, LarissaVanni, Tazioda Graa Salomo, MariaProbst, Livia FernandesViscondi, Juliana Yukari K.BioMed CentralBMC Infectious Diseases...
Following purification, nanoparticle quality and concentration was first measured by UV-vis spectroscopy. Nanoparticle polydispersity and purity was then assessed using SDS-PAGE, DLS, and nsEM. Finally, endotoxin levels were measured using the LAL assay, with all immunogens used in animal studies cont...
View PDFView articleGoogle Scholar World Health Organization, 2023a World Health Organization Summary of neuraminidase (NA) amino acid substitutions associated with reduced inhibition by neuraminidase inhibitors (NAIs) Updated March 7, 2023. Available at: https://www.who.int/publications/m/item/summary...
A comparative analysis of the full sequences of the eight gene segments confirmed that the Chilean viruses had a distinct nature vis-a-vis other AIVs, forming a unique clade exclusive to South America. These findings highlight the critical importance of the continuous moni- toring and ...