significant increase in total number of T cells after JEV infection. Notably, the CD4+ T cells were found to be more enriched than CD8+ T cells (Fig.2F, G) [45,46]. To further confirm these findings, the IHC assay was performed, and consistent changes on Mon_Mac, Mic_Mac, T ...
Furthermore, we used TUNEL assay to detect neuronal damage in each group. There were more TUNEL-positive neurons in the hippocampal CA1 and CA3 regions of APP + PTZ mice compared with APP + SA (Fig. 4G). These results suggested increased neuronal damage in PTZ-kindling APP mice,...
Real-time quantitative PCR assay (Fig. 2E) and western blotting assay (Fig. 2F) (Supplementary Fig. 2A) indicated a reduced production of ARG1 in M2-like macrophages when treated with GDNPs. ELISA results, which measured ARG1 concentration in the supernatant of different phenotypes, showed that...
Western blot Liver tissue lysates were homogenized in RIPA lysis and extraction buffer containing protease inhibitors (Millipore, Boston, USA). Total protein was quantified using the BCA Protein Assay Kit (GBCBIO, Guangzhou, China), and equal amounts of protein were separated by SDS-PAGE and trans...
5a; P = 0.031) and the LDH assay revealed increased LDH release in STHdh Q111/Q111 compared to STHdh Q7/Q7 cells (Fig. 5b; P = 0.022), suggesting that mutant cells are characterized by reduced viability and increased cell death, in contrast to the first findings. ...
chx for 0h, 0.5h, 1h, 2h, 4h and 6h. then, the cells were harvested, lysed with ripa buffer and then applied for western blottingto measure the bfgf protein level. ubiquitination assay the cells were treated with the proteasome inhibitor mg132 (0.5μm) for 6h and lysed with ...
The most common site of metastasis in breast cancer is the bone, where the balance between osteoclast-mediated bone resorption and osteoblast-mediated bone formation is disrupted. This imbalance causes osteolytic bone metastasis in breast cancer, which l
Western blot For Western blot experiments, equivalent amounts, determined by a bicinchoninic acid (BCA) assay, of protein were separated by SDS-PAGE and subsequently electroblotted onto polyvinyldifluoride (PVDF) membrane. The PVDF sheets were blocked with 3% bovine serum albumine (BSA) in TBS (...
Previous in vitro study on the differentiation of primary human bone marrow-derived mesenchymal stem cells (PCS-500-012TM, ATCC) on 3-D SiO2/PTHF/PCL-diCOOH scaffolds with specific channel sizes have been investigated. These analyses revealed that a channel width of 200–250 µm was optimal...
2.3. MTT Assay The effect of the essential oil from C. ambrosioides on cell viability was detected by using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. In short, L02 cells were seeded at a density of 1 × 104 cells/well in a 100 μL volume of...