It is critical to understand the degree of cell death in any flow cytometry assay and exclude those cells from the analysis. BioLegend provides DNA dyes, Propidium Iodide and 7-AAD, that enter and stain dead cells, but are impermeable to live cells for r
The cell clustering approaches can be categorized into gene expression-based and genotype-based approaches. Gene expression-based approaches regard each cluster as a unique cell type or sub-population of a known cell type with a distinct expression pattern. However, the high-dimensionality of single-...
Part 1. Introduction Morphology of cells in culture Cells in culture can be divided in to three basic categories based on their shape and appearance (i.e., morphology). • Fibroblastic (or fibroblast-like) cells are bipolar or multipolar, have elongated shapes, and grow attched to a ...
However, development of a valid potency assay for stem cell product lot release will be required for FDA licensure. It is generally expected that a potency assay will be in place before phase 3. An expanded discussion of this important topic is presented in the subsection on potency in the ...
Chemotactic extravasation and metastatic colony formation are regulated by PAK1.aScratch wound migration assay. Cells were plated and grown to confluency. A wound was manually made and cell motility was monitored every 4 h.bChemotactic cell migration. Cells were serum starved and subjected to tran...
assay cell proliferation in individual cells or in cell populations. The rate of new DNA synthesis can be based on incorporation of a nucleoside analog such as BrdU or EdU into DNA. Detection of these two thymidine analogs varies widely and has implications on the assay results and m...
The hair follicle cycling assay was conducted according to a previously reported guideline73. Assay for liver fibrosis (LF) in mice Liver fibrosis induction was conducted as previously described74,75. Male C57BL/6J mice (8 weeks old) were randomly divided into three groups as follows (n =...
This work presents a head-to-head comparison of prognostic methylation markers for RCC using a novel approach to determine the optimal location for DNA methylation-based biomarker development. We demonstrated that using epigenomic TCGA data to guide methylation assay design is a suitable approach to ...
Severe dengue (SD) is a major cause of morbidity and mortality. To define dengue virus (DENV) target cells and immunological hallmarks of SD progression in children’s blood, we integrated two single-cell approaches capturing cellular and viral elements:
BBζ CAR T cells showed no macroscopic disease and ex vivo BLI of organs did not reveal any signal (Supplementary Fig.10b), suggesting that these mice were likely cured. We identified peripheral persistence of STEAP1-BBζ CAR T cells at the end of the experiment based on the presence of ...