In Cell Western Protocol Odyssey 近红外双色激光成像系统 In-Cell Western Protocol EGF对A431细胞刺激效应的评估 （中文版）基因有限公司 2007年1月
(1) long passage HeLa cells are not suitable for the assay because of high basal level phosphorylation of ERK. As a result, ordering a new sample of this cell line from ATCC is highly recommended. (2) No starvation is needed or recommended. II. Sample Protocol...
In-Cell Western Assay Overview Kit Components IRDye®800CW Goat anti-Rabbit Secondary Antibody, 0.5 mg IRDye 680RD Goat anti-Mouse Secondary Antibody, 0.5 mg Intercept®(TBS) Blocking Buffer, 1 x 500 mL CellTag 520 Stain, 2 x 50 nmol ...
Krisztina Papp-Wallace (Veterans Affairs Northeast Ohio Healthcare System and Case Western Reserve University) for advice on the biochemistry of peptidoglycan recycling; the Donnelly Centre (Toronto, Canada), The Centre for Applied Genomics (Toronto, Canada), and Génome Québec (Montréal, Canada) ...
Western blot with SARS-CoV-2-specific polyclonal antibodies was used to detect the bands at 20–25 kDa. (C) Confirmation of surface display of SARS-CoV-2 antigens on L. plantarum, determined using immunofluorescence microscopy Full size image Further, surface expression of the SARS-CoV-2 ...
(FRET) Microscopy PDF Unit 17.2 Identification of Protein Interactions by Far Western Analysis PDF Unit 17.3 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins PDF Unit 17.4 Mapping Protein-Protein Interactions with Phage-Displayed Combinatorial Peptide Libraries PDF Unit 17.5 Protein-...
Western blots Cultured cells were washed twice in PBS and lysed in RIPA buffer for 30 min on an orbital shaker at 4 °C. Subsequently, the cell lysate was centrifuged for 20 min at 20,000g. The cleared lysate was transferred to a new tube and total protein was quantified by BCA...
To confirm the bioinformatics-predicted target gene of miR-340-5p, western blotting and RT-PCR were performed. The results revealed that the KMT5A expression level was significantly downregulated by the forced expression of miR-340-5p compared with its negative control, the m-NC-transfected cells...
The CAT protein expressed from the pcDNA5/FRT/CAT control plasmid is approximately 32 kDa in size. You may assay for CAT expression using your method of choice. For Western blot analysis, you may use CAT Antiserum availab...
Antibodies used for Western blot (WB) and immunohistochemistry analyses (IHC); Table S3. Antibodies used for multiplex immunofluorescence (mIF); Table S4. Association between the protein expression of ACSL6 and the clinicopathological parameters in primary lung tumors; Figure S1. Tissue distribution ...