Cytotoxic T Lymphocytes In Vivo 1Andrew, Kate ASimkins, Helen M AWitzel, SabineHudson, JennyHermans, Ian FRitchie, David SPerret, RachelYang, JianpingRonchese, Franca
Cytotoxic CD8+ T cells are responsible for the lysis of cells expressing peptides associated with MHC class I molecules and derived from infection with a p... N Chaoul,C Fayolle,C Leclerc - BIO-PROTOCOL 被引量: 0发表: 2016年 In vivo OVA-specific Cytotoxic CD8+ T Cell Killing Assay Cytot...
Human cytomegalovirus (HCMV)-specific CD8(+) cytotoxic T lymphocytes (CTL) appear to play an important role in the control of virus replication and in protection against HCMV-related disease. We have previously reported high frequencies of memory CTL precursors (CTLp) specific to the HCMV tegum...
(a–c) Analyses of PDIA3 expression signatures linked to in cytotoxic T lymphocyte (CTL) – associated survival benefits in patients, where high-level of PDIA3 abolishes or weakens the overall survival benefit of CTL-high patients with GBM (a), TNBC (b), or LUAD (c). X-axis is overal...
Cytotoxic T-lymphocyte assay In order to evaluate the mechanism of the immune response, an in vitro CTL assay was performed 3 d after conclusion of GCV treatment in the HSV-tk/IL-2 treated group as described previously (Chen et al. 1995). Briefly, splenocytes were isolated from nonmelanom...
CD8+ cytotoxic T cells (CTLs) orchestrate antitumour immunity and exhibit inherent heterogeneity1,2, with precursor exhausted T (Tpex) cells but not terminally exhausted T (Tex) cells capable of responding to existing immunotherapies3–7. The gen
We found no significant correlation between in vivo CAR–T-cell expansion/persistence and the following: CAR–T-cell dose; time for ex vivo culture; fold expansion ex vivo; cytotoxic activity ex vivo; proportion of CD4+or CD8+T-cell subsets, or CD45RO, CD62L, or CD28 expression in CAR...
3b, c). The effect of the combination of PAA-TiOxNPs and X-ray irradiation evaluated by a colony forming assay was significantly better than that of X-rays alone (p < 0.05, Fig. 3d). Overall, the PAA-TiOxNPs enhanced the cytotoxic effect of X-ray irradiation in vitro. Fig. 3 ...
Since Ccl3-OVA failed to induce OT-I proliferation when incubated with cDC1 in vitro we wanted to test if Ccl3-HA induced cytotoxic T cell responses in the absence of cDC1. We therefore repeated the cytotoxicity assay after i.m. immunization with Xcl1-HA or Ccl3-HA in BATF3−/−...
contribute to the thyroidal tissue microenvironment in HT. These cell types occupy distinct histological locations within the thyroid gland. Our experiments suggest that they might facilitate lymphocyte trafficking from the blood to thyroid tissues, and T cell zone CCL21+fibroblasts may also promote the...