3D assayimage processing toolsin vitroscratch assayskin modelWound healing is essential for the restoration of the barrier function of the skin. During this process, cells at the wound edges proliferate and migrate, leading to re-epithelialization of the wound surface. Wound healing assays are used...
Wound healing is a very complicated process. In this study, an in vitro tube formation assay, a wound healing assay, and a cell proliferation test were carried out to examine the activity of “Jinchuang ointment.” Here, we report the outcomes of treating patients with “Jinchuang ointment,”...
The aim of this study is to comparein-vitroandex-vivotest systems for their usability for early drug screening and to investigate the efficacy of a birch bark triterpene extract (TE) that has been provenex-vivoand clinically to accelerate non-diabetic wound healing (WH), in a diabetic ...
The detection was performed using the Cell-Light EdU-594 in vitro imaging kit (RiboBio, Guangzhou, China) according to the manufacturer’s protocol. Used a fluorescence microscope to capture the image. The results were analyzed by two blinded observers. Dual-luciferase reporter assay After sequence...
Furthermore, the present report becomes a pilot study in transferring from in vitro to in vivo scale (e.g., medical field application) once LBPC-AgNCs have demonstrated a unique wound healing potential as well as a non-toxic effect on the liver and blood. Keywords: mouse model; silver ...
The methanolic extract of SP was tested for the ability to stimulate the growth of mouse fibroblast NIH3T3 in vitro. The viability and proliferation of fibroblasts were evaluated at 72 h after cell seeding by MTT assay at 570 nm. To study wound healing properties in vivo, excision and incisio...
2B). The migration of 4T1 cells was assessed by wound healing assay at 24 and 48 h. Both sevoflurane and propofol suppressed the migration of 4T1 cells in a dose dependent manner (Supplementary Fig. 2C, D). Thus, the in vitro effects of both anesthetics on 4T1 cells do not seem ...
This study explores the effects of halibut oil cream on different phases of wound healing and assess its potential as a supplement for promoting wound heal
Fig. S4 Pro-healing cytokine secretion in macrophages stimulated by piezoelectric hydrogels. Fig. S5 Representative quantitative analysis of the migratory ability of HUVEC cells in the Transwell (a) and wound healing migration (b) assay for Fig. 4b-c. Fig. S6 In vitro evaluation of osteogenic ...
Cultures of epithelial cells are limited by the proliferative capacity of primary cells and cell senescence. Herein we show that primary human epithelial cell sheets cultured without dermal equivalents maintained homeostasis in vitro for at least 1 year.