Using Live/Dead assay, most of the ADSCs on the ADM under normoxic or hypoxic condition were positive for green fluorescence (live cells), while a few of ADSCs were positive for red fluorescence (dead cells). Statistically, the viability of ADSCs on the ADM membrane cultured under normoxic...
The migration rates of cells were determined by the wound healing assay and transwell assay. As for the wound healing assay, cells were plated into 12-well plates (Corning) at a density of 2.5 × 105 cells per well, and continuous culture occurs until the cell density reaches above 90%. ...
2.5. In Vitro Wound Healing Assay HCECs were seeded into six-well plates and grown to confluence. The monolayer was scratched using a 200 μl pipette tip and washed with serum-free medium to remove detached cells. Then, the cells were kept in coculture with HUMSC-sEVs or not. At diff...
In next study, we used wound healing and cell migration assay to examine that whether HGF functions were affected after ILK gene depletion. Figure 3 (a) Open in figure viewerPowerPoint HGF promotes ILK and c-met expression in scratched epidermal cells. (a) ILK and c-met expression in ...
To see if MDL-800 can promote cell migration, we performed the transwell migration assay and used crystal violet staining to track the cell migration through the transwell membrane. The results (Figures 2(a) and 2(b)) showed that the 2.5 μM MDL-800 group had the most stained HUVECs,...
2.4. Cell Proliferation Assay Human neonatal epidermal keratinocytes (HEKn) were purchased from Cascade Biologics and cultured in complete serum-free keratinocyte medium (K-SFM) containing recombinant epidermal growth factor (rEGF) and bovine pituitary extract (BPE) (Invitrogen). HEKn cells (4,000)...