awill actually be prescribed macrolides 意志实际上是被规定的大环内酯[translate] a技术部门仍在处理中 Technical department still in processing[translate] aIn vitro pull-down assay of the recombinant SlCIPK2 showed that 再组合SlCIPK2的体外拉下来分析用试样显示了那[translate]...
aIn vitro pull-down assay of the recombinant SlCIPK2 showed that SlCIPK2 interacts with SlCBLs and stress-responsive transcription factors, SlERF7, SlCBF1 and SlAREB1. 再组合SlCIPK2的体外拉下来分析用试样表示, SlCIPK2与SlCBLs和注重敏感副本因素, SlERF7、SlCBF1和SlAREB1互动。[translate]...
The in vitro pull-down assay is a well-established method to confirm direct binding in protein-protein interactions that was inferred from other interaction assays, such as two-hybrid analysis (1). The assay is usually carried out using glutathione-S-transfer ase-tagged or His-tagged fusion pro...
In Vitro HisTag Pull-Down Assay Using MagZ™ ParticlesBecky GodatTonny Johnson
2b). An in vitro pull-down assay was performed to verify that 230D7 dose-dependently reduces PTEN binding to SPOPMATH (Supplementary Fig. 2c, d). After validating the molecular activity, we used 230D7 for the functional study at the cellular level....
(QDO) but containing X-α-Gal for 3 days at 30 °C. The pGBD-P53 + pGAD-SV40 combination was used as a positive control. X-α-Gal represents 5-bromo-4-chloro-3-indolyl-α-D-galactoside.bIn vitro pull-down assays showing the interaction of OsUBC12 with OsSnRK1.1. His-tagged ...
文档标签: 2017年整理In vitro pull down 系统标签: 诱饵 蛋白 vitro gtpase 蛋白质 猎物 Pull-Down技术是通过蛋白相互作用来研究细胞通路的有力工具。Pull-Down实验是确定两种或更多蛋白之间相互作用的体外方法。Pull-Down实验可用来检测已知蛋白的表达和相互作用条件,并且可用来筛选未知的蛋白相互作用。Pull-Down实验...
In vitro pull down Pull-Down技术是通过蛋白相互作用来研究细胞通路的有力工具。Pull-Down实验是确定两种或更多蛋白之间相互作用的体外方法。Pull-Down实验可用来检测已知蛋白的表达和相互作用条件，并且可用来筛选未知的蛋白相互作用。Pull-Down实验至少需要一种纯化的标签蛋白（诱饵）用来捕获和“pull-down”靶蛋白（...
Interestingly, it was recently reported that CRHSP-28 specifically interacts with a member of the MAL protein family by yeast two-hybrid analysis and in vitro pull-down assays (12). MAL proteins are known to regulate apical targeting of vesicles in renal epithelial cells and, similar to annexin...
In vitro RNA-protein pulldown assays 3’-biotin-labeled ssRNA (5’-CGUCUCGGACUCGGACUGCU-3’) and complementary ssDNA (5’-AGCAGTCCGAGTCCGAGACG-3’) were synthesized from TsingKe Biotech Co., Ltd (Beijing, China). We generated m6A ssRNA by substituting all two adenines with m6A-modified ...