In vitro metabolic engineering refers to the assembly of multiple purified/partially purified enzymes as well as nonenzyme components such as cofactors according to the designed pathways for the conversion of certain substrates into target compounds without the utilization of cells. Resembling an ...
Fig. 1. Summary of in vitro metabolic engineering (ME) approaches. 1. In vivo metabolic engineering, in which model microorganisms like Escherichia coli and Saccharomyces cerevisiae are often accompanied with inefficient and time-consuming pathways construction, transformation and fermentation; 2. Cell-...
Thus, strain 0019 was used thereafter for metabolic engineering of acetyl-CoA supply from different carbon sources. Fig. 2 Effects of N-acetylglutamate synthase (NAGS) from different species on NAG production. a Effects of NAGS reported in references on NAG production. b Effects of NAGS not ...
Corneal endothelial cells (CECs) are very important for the maintenance of corneal transparency. However, in vitro, CECs display limited proliferation and loss of phenotype via endothelial to mesenchymal transformation (EMT) and cellular senescence. In t
studies in which the SAR was measured alone, since using this ratio would imply similar PD values to those we have considered. The high SAR to PD ratio implies that it is difficult to apply findings from in vitro or in vivo experiments to human exposure situations, since PDs at the limit...
Liver-derived cell lines from cavefish Astyanax mexicanus as an in vitro model for studying metabolic adaptation. Sci Rep. 2022;12(1):10115. (Open in a new window)PubMed (Open in a new window)Web of Science ®(Open in a new window)Google Scholar Takahashi K, Yamanaka S. Induction...
This mini-review summarizes the recent progresses on the emerging topic of in vitro metabolic engineering and comments on the potential application of cell-free technology to speed up the "design-build-test" cycles of biomanufacturing.doi:10.1016/j.csbj.2017.01.006Weihua Guo...
Novel chemical entities can be prepared from these artificial pathways.doi:US7427497 B2DORDICK JONATHAN S.SRINIVASAN ARAVINDKIM JUNGBAESHERMAN DAVID H.CLARK DOUGLAS S.US
metabolic engineering. Expression of a gene on the pCC5.2 plasmid leads to higher expression level than that on the chromosome as well as the RSF1010 self-replicating plasmid26. Furthermore, during the stationary phase of cell growth, the copy numbers of the endogenous plasmids (pCA2.4, pCB2.4...
Numerous drugs and drug precursors in the current pharmacopoeia originate from plant sources. The limited yield of some bioactive compounds in plant tissues, however, presents a significant challenge for large-scale drug development. Metabolic engineerin