In vitro Rev-RRE binding assay.Francesc CunyatNancy BeerensElisabet GarciaBonaventura ClotetJørgen KjemsCecilia Cabrera
application of the HEV-binding assay to a clinical series High endothelial venule (HEV)-binding of peripheral blood mononuclear cells (PBMCs) from 43 patients with B-cell chronic lymphocyticleukaemia (B-CLL) was i... GyÖrgy,Csanaky,Veronika,... - 《Medical Oncology》 被引量: 15发表: ...
contributed to in vitro binding assay; S.C. contributed to ChIP experiments with transient transfection. X.W., F.Z. and P.A.S. wrote the manuscript with help from all other authors. R.J., F.Z. and P.A.S. supervised the research. Corresponding authors Correspondence to Feng Zhang or...
Protocol Published: 15 March 2007 In vitro screening for molecules that affect virus capsid assembly (and other protein association reactions)Adam Zlotnick, Angela Lee, Christina R Bourne, Jennifer M Johnson, Paul L Domanico & Stephen J Stray ...
Medicilon's cell viability assay platform can provide compliant in vitro cell viability detection services that meet the application requirements.
leading to the development of resistance (Fig.6K). Likewise, SubMap also revealed patients with high COL8A1-ITGB1 score were more likely to be non-sensitive to chemotherapy (Fig.6L). To experimentally validate our observations, in vitro culture of CRC cells with COL8A1 or siITGB1 revealed ...
Here, we provide a protocol for the selection of conformation-specific intracellular antibody degraders using a cell-based screening method. We applied this protocol to select antibody-based degraders targeting the active form of the small GTPase RHOB (i.e., RHOB-GTP) using an engineered H2882 ...
Common methods and its advantages for protein kinase assay Profacgen offers: 1. Radioisotope method : including membrane filtration and close scintillation counting. Protocol: Prepare the kinase substate to capture on the plate surface by the capture agent. The kinase and isotopically-labled ATP ...
The effect of nanoparticle size on the probability to cross the blood-brain barrier: an in-vitro endothelial cell model. J. Nanobiotechnology, 10.1186/s12951-015-0075-7 (2015). Zhang, G. et al. Influence of anchoring ligands and particle size on the colloidal stability and in vivo ...
NK and CD4+T cell cocultures in vitro CD4+T cells were cultured at 1 × 106cells per ml in C10 media with 20 ng per ml of IL-2 and Gibco Dynabeads Human T-Activator CD3/CD28 (Thermofisher) per manufacturer protocol for 3 days. Then co-stimulated CD4+T cells were resuspended...