We further explored the effect of depletion of the proteins that bind these motifs on chromosome organisation by performing siRNA mediated depletion of Beaf-32, M1BP or both, followed by in situ Hi-C protocol (Methods section). We used the knockdown of GST as a negative control and two ...
The probe systems include hydrogen induced cracking (HIC)-resistant microstructure such that as atomic hydrogen permeates the probe surface, the probe captures recombined hydrogen gas. The pressure of the resultant hydrogen gas buildup is measured and predictions as to the HIC activity of that area...
In situ Hi-C was performed on two independently prepared biological replicates using a previously published protocol54. Read mapping, as well as filtering and iterative correction, were performed using scripts derived from those at https://bitbucket.org/mirnylab/hiclib. The mapped Hi-C fragments ...
One strong (red, originating from goldfish) and one weak (white, originating from common carp) signal were detected in the intergeneric F1 using fluorescence in situ hybridization, while two strong (red) and two weak (white) signals were observed in allotetraploid F22 and F24 (Scale bar: 3 ...
Ferroptosis, a therapeutic strategy for tumours, is a regulated cell death characterised by the increased accumulation of iron-dependent lipid peroxides (LPO). Tumour-associated long non-coding RNAs (lncRNAs), when combined with traditional anti-cancer m
Of note, the equivalent to second line oxaliplatin reintroduction attempted in the OPTIMOX trials was considered unsuccessful by the authors, not for lack of activity but rather because of feasibility issue (too many protocol violations) [42]. We were unable to reliably reconstruct the oxaliplatin-...
To cause HIC, the surface must absorb hydrogen at the interface of metal/oxide, which causes embrittlement [78]. In this research, hydrogen in the H2SO4 electrolyte generates H+ ions that can sensitize the anodizing, provoking a faster dissolution. For that reason, some parts of the anodized...
14, 15, 16 Because membrane blebbing can produce a type of microvesicle large enough to be detected by methods other than electron microscopy or biochemical isolation, the question we address herein is whether such a large tumor-derived vesicle can be observed and quantified in situ in tumor ...
using NucBlue Fixed Cell ready probes that were added for 10 min followed by rinsing with tap water. The samples were cut into 4 μm slides. The sections were then stained with H&E as instructed. The cell death analysis was performed using the TUNEL kit as instructed by the protocol. ...
Fluorescence in situ hybridization The FISH assay followed the RNA-FISH kit protocol (GenePharma, Suzhou, China). Cells were seeded on glass slides in 24-well plates, fixed with 4% paraformaldehyde, and permeabilized with 0.3% Triton X-100. After 30 min of blocking at 37℃, they were hybrid...