Prometaphase chromosome analysis was undertaken in a patient with familial tricho-rhino-phalangeal syndrome type I (TRPS-I). The patient had apparently normal chromosomes and produced counterevidence to part of
(referred to as ecDNA and visible as double minute chromosomes during metaphase12). Despite our increasing ability to characterize multiple features in single cells13, an in-depth characterization of circular DNA content, structure and sequence in single cells remains elusive with current approaches. ...
Accurate chromosome segregation during meiosis is crucial for reproduction. Human and porcine oocytes transiently cluster their chromosomes before the onset of spindle assembly and subsequent chromosome segregation. The mechanism and function of chromoso
. Serine 31 phosphorylation is unique to histone H3.3 and was originally identified to be localized adjacent to centromeres in metaphase chromosomes [14]. It is also a mitosis-specific marker different from H3 S10P and S28P in terms of timing and localization [14]. Banaszynski’s group rece...
which cannot be physically separated from each other. This is possible only during the metaphase stage of cell division when the chromosomes are condensed. Early studies isolated single chromosomes from metaphase spreads of dividing cells using a micromanipulator (Chambers and Sands1923; Barigozzi1939;...
Primed in situ hybridization using primers for crasiRNA pool sequence, SINE28 (green), to tammar metaphase chromosomes (grey). SINE28 sequences are found localized to the tammar centromeres. Full size image While our previous study showed that the original pool of small RNAs in the size range...
B, exhibition of the morphology of metaphase chromosomes in HeLa cells after overexpressing GFP, full-length apoa2-GFP, and GFP-fused apoa2 C-terminal mutant MC46. Metaphase chromosomes separated mutually (a–b) in control and fused morbidly (c–f) in the case of loss or gain of the ...
Therefore, in order to determine whether HD-induced TE activation can impact genomic stability, we analyzed metaphase chromosomes obtained from elav-Gal4>128QHtt larval brains and control ones (elav-Gal4/+), showing that HD metaphases present abnormal chromosome configurations such as breakages, ...
b, Assembly of the high-copy focal amplification amp2 from the same data. All junctions are supported except a peritelomeric region adjacent to the gap, which was inferred from WGS discordant reads only. c, Metaphase FISH microscopy targeting amplified DNTTIP2 (amp1), KMT2E (amp2) and ETV...
Article 23 September 2024 PAK2 is essential for chromosome alignment in metaphase I oocytes Article Open access 22 February 2023 Prc1-rich kinetochores are required for error-free acentrosomal spindle bipolarization during meiosis I in mouse oocytes Article Open access 27 May 2020 Introduction...