RNA which is resistant in situ to ribonuclease in solutions of high ionic strength was demonstrated in interphase nuclei and metaphase chromosomes of human PHA-blasts. Since the synthesis of this RNase-resistant RNA is not affected by actinomycin D it is likely that it is of a non-ribosomal ty...
Chowdhary P, De la sene C, Harbit I, Eriksson l and Gustavsson I (1995) FISH on metaphase and interphase chromosomes demonstrates the physical order of the genes for GPI, CRC and LIPE in pigs. Cytogenet. Cell Genet. 71, 175-178....
A checkpoint mechanism known as the SAC delays the separation of the sister chromatids at anaphase until every kinetochore has correctly attached to spindle microtubules and all sister chromatids have aligned at the metaphase equatorial plate.185,186,187,188Thus, the SAC is a safeguard for guarante...
S. Alterations in metaphase durations in cells derived from human tumours. Cell Prolif. 18, 137–146 (1985). CAS Google Scholar Therman, E., Buchler, D. A., Nieminen, U. & Timonen, S. Mitotic modifications and aberrations in human cervical cancer. Cancer Genet. Cytogenet. 11, 185–...
Subsequently, the algae pellets were fixed with a solution of 3 parts ethanol to 1 part acetic acid (v/v) for 24 h to ensure fixation of the algae cells in the metaphase of mitosis. After fixation, the cells were washed three times with distilled water and then subjected to enzymatic ...
Metaphase spread analysis of ploidy and chromosomal aberrations Cells were plated in 10 cm dish, grown to 70% confluence and treated with Colchicine in culture at 1.0 μg/mL for primary liver cancer cell line, 0.8 μg/mL for NIH-3T3 and Hepa1-6 cells and cells for 6 h. Cells were the...
metaphase of the first meiotic divisions, individual univalents, bivalents, and multivalents cannot properly attach to the spindle and segregate, causing the spermatocytes to become arrested at this stage of meiosis. Chromosomes ofI. longicorpaare marked in orange; chromosomes ofC. hankugensis are...
G2 phases and become blocked at metaphase by the absence of spindle microtubules. Deletion of eithermad2ormad3was additive to thepmk1Δmutant, as cells leaked from mitosis with faster kinetics than the respective single deletions (Fig.1I, J). Theslp1-mr63allele is defective in Mad2 ...
2c and Extended Data Fig. 3c). We next examined how inhibiting KIF18A affects the mitotic progression of HeLa cells. We determined that HeLa cells were sensitive to KIF18A-inhibitor treatment and shifted the localization of KIF18A protein from the equatorial region sandwiching the metaphase plate...
(referred to as ecDNA and visible as double minute chromosomes during metaphase12). Despite our increasing ability to characterize multiple features in single cells13, an in-depth characterization of circular DNA content, structure and sequence in single cells remains elusive with current approaches. ...