In metaphase (a), the microtubules of the spindle (white) have attached and the chromosomes have lined up on the metaphase plate. During anaphase (b), the sister chromatids are pulled apart and move toward opposite poles of the cell.
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Metaphaseis the third of the five phases of biological cell division, or more specifically, the division of what is inside that cell's nucleus. In most instances, this division ismitosis, which is the means by which living cells duplicate their genetic material (DNA, or deoxyribonucleic acid, ...
Metaphase chromosomes released from cells in the presence of Joklik's suspension media by vortex-mixing with 0.5 mm glass beads have been analyzed by electron microscopy. In these preparations the chromosomes are composed of series of loops (200–300 Å in diameter) which are, in turn, compos...
During interphase, chromosomes are not distinctly visible as they are in the form of chromatin, which is less condensed. - Prophase: In this phase, chromosomes begin to condense and become visible. However, they are still not as distinct as they will be later. - Metaphase: During metaphase,...
. Serine 31 phosphorylation is unique to histone H3.3 and was originally identified to be localized adjacent to centromeres in metaphase chromosomes [14]. It is also a mitosis-specific marker different from H3 S10P and S28P in terms of timing and localization [14]. Banaszynski’s group ...
Cells were concentrated in metaphase by treatment with KaryoMAX Colcemid (Gibco) at 100 ng ml−1for between 3 h and overnight (depending on the cell cycle speed). Cells were washed once with PBS and a single-cell suspension was incubated in 75 mM KCl for 15 min at 37 °...
Primed in situ hybridization using primers for crasiRNA pool sequence, SINE28 (green), to tammar metaphase chromosomes (grey). SINE28 sequences are found localized to the tammar centromeres. Full size image While our previous study showed that the original pool of small RNAs in the size range...
The spatial folding of chromosomes inside the nucleus has regulatory effects on gene expression, yet the impact of genome reshuffling on this organization remains unclear. Here, we take advantage of chromosome conformation capture in combination with single-nucleotide polymorphism (SNP) genotyping and ana...
synapsis.Successful formation of the protein complex between paired homologou chromosomes is required for the cell to proceed into metaphase of mitosis.4 Dynein is necessary to ensure proper synapsis of homologous chromosomes.D A 2,3 and 4 only B 1,2 and 4 only C 1and 3 only D 2 and 4...