compared. Proteins from cells immediately before and 30 min after the addition of 0.2 mM H2O2were separated by native PAGE and stained for catalase activity. Again, the wild-type and ΔarcAstrains exhibited abilities comparable to each other w/o the H2O2treatment whereas theoxyRmutant was ...
Additionally, one line harboring an in-frame mutation, Mpnop1-22 ge-i, was also further analyzed. All six lines retained the knockout mutant phenotype and exhibited no reversions or additional phenotypes in the four analyzed gemmae generations (Fig. 3e, f). Consistency of the unaltered target...
PTENα remains active in PTEN-mutant cancer Loss-of-function mutations in the PTEN gene are frequent in human cancer3. In addition to phosphatase-inactive mutations, abundant stop-gained mutations were detected in exons encoding the C-terminal domain of PTEN (Fig. 1a). Through induction of PTEN...
Mutant construction and enzymatic assays The mutants of PnGH1 were obtained using the Fast Mutagenesis system (Transgen Biotech, Beijing, China). Primers used are shown in Supplementary Table 4. The heterologous expression, protein purification, and enzyme assay procedures were the same as described ...
Liu J, Zhu JK (1997) Proline accumulation and salt-stress induced gene expression in a salt- hypersensitive mutant of Arabidopsis. Plant Physiol 114(2):591–596 Article CAS PubMed PubMed Central Google Scholar Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki K, Shinozaki...
Table 4 Enzyme activities in the control and Δpta mutant strain. Full size table When considering the acetate metabolism, Acs was only detected in the stationary phase of both strains, although expression in the Δpta mutant was three-fold lower, probably as a result of low acetate levels (...
The double aroK-aroL- mutant expressing plasmid-coded genes (strain PB12.SA22) accumulated SA up to 7 g/L with a yield of SA on glucose of 0.29 mol/mol and a total aromatic compound yield (TACY) of 0.38 mol/mol. Single inactivation of pykF or pykA was performed in PB12.SA22 ...
To elucidate the function ofmamXin the absence of polar effect, MSR-1 was subjected to in-frame gene deletion (to produce strain ∆mamX) and complementation ofmamX(to produce strain CmamX) as described in Methods. We validated the construction of the mutant and complemented strains, detecte...
(references are in Supplementary Table14). U: ubiquitynation; N: S-nitrosylation; O: oxidation; Ac: acetylation; S: sumoylation; P: phosphorylation. Exon structure is indicated above protein. M/I, missense or inframe indel. T, truncating mutation (frameshift, nonsense). NLS: Nuclear ...
Among various mutant detection methods NGS was the most informative and reliable assay to genotype the KO individuals. The deep sequencing analysis of the resultant genotypes suggested that while the total mutation and frame-shift mutation rates did not clearly correlate with the observed phenotypes, ...